C166细胞
ATCC Number： CRL-2581™
细胞形态： 内皮样
细胞类型： 内皮细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
生长状态： 贴壁生长
数量： 大量
年限： 12 day embryo
器官来源： 其他
Designations: C166
Depositors: R Auerbach
C166细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: endothelial
Source: Organ: yolk sac
Cell Type: endothelial
Cellular Products: angiotensin converting enzyme (ACE) [51511]
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Applications: The C166 cell line was established from cells from F1 embryos obtained by mating a female NMRI/GSF mouse with a male CD-1 mouse that was transgenic for the human fes (fps/fes) proto-oncogene.
C166 cells exhibit normal endothelial characteristics, C166细胞such as rearrangement into tubelike structures when placed on Matrigel and retention of cobblestone morphology at confluence.
The cell line expresses high levels of the cytoplasmic protein-tyrosine kinase encoded by the fes (fps/fes) proto-oncogene.
The cell line should prove useful for the study of endothelial cell differentiation and for the determination of the mechanisms underlying the establishment of organ-specific endothelial cell heterogeneity.
The cell line can support the stable proliferation of multipotential hematopoietic stem cells, thus generating adequate numbers of cells for study of the mechanisms involved in their subsequent development and differentiation.
Receptors: acetylated low density lipoprotein (LDP) [51511]
Oncogene: fes + (fps/fes +)
Antigen Expression: vascular cell adhesion molecule 1 (CD106, VCAM-1) [51511]
vascular addressin + [51511]
Age: 12 day embryo
Comments: The C166 cell line was established from cells from F1 embryos obtained by mating a female NMRI/GSF mouse with a male CD-1 mouse that was transgenic for the human fes (fps/fes) proto-oncogene. [51511]
These mice express multiple copies of an activated allele of the human fes (fps/fes) proto-oncogene and display hypervascularity progressing to multifocal hemangiomas. C166细胞[51511]
C166 cells exhibit normal endothelial characteristics, such as rearrangement into tubelike structures when placed on Matrigel and retention of cobblestone morphology at confluence. [51511]
The cells constitutively express the vascular addressin identified by antibody MECA-99. [51511]
The cell line expresses high levels of the cytoplasmic protein-tyrosine kinase encoded by the fes (fps/fes) proto-oncogene. [51511]
The cell line should prove useful for the study of endothelial cell differentiation and for the determination of the mechanisms underlying the establishment of organ-specific endothelial cell heterogeneity. [51511]
The cell line can support the stable proliferation of multipotential hematopoietic stem cells, thus generating adequate numbers of cells for study of the mechanisms involved in their subsequent development and differentiation. [51515]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
C166细胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2582
derivative:ATCC CRL-2583
References: 51511: Wang SJ, et al. Isolation and propagation of yolk-sac-derived endothelial cells from a hypervascular transgenic mouse expressing a gain-of-function fps/fes proto-oncogene. In Vitro Cell. Dev. Biol. Anim. 32: 292-299, 1996. PubMed: 8792159
51515: Lu LS, et al. In vitro and in vivo differentiation into B cells, C166细胞T cells, and myeloid cells of primitive yolk sac hematopoietic precursor cells expanded > 100-fold by coculture with a clonal yolk sac endothelial cell line. Proc. Natl. Acad. Sci. USA 93: 14782-14787, 1996. PubMed: 8962132
