2H-11细胞
品系： C3H/HeJ
年限： *****
组织来源： vascular epithelium
ATCC Number： CRL-2163™
运输方式： 冻存运输
数量： 大量
细胞类型： SV40转化细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： 上皮样
生长状态： 贴壁生长
器官来源： 腋窝**结
Designations: 2H-11
Depositors: KA O'Connell
2H-11细胞Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: axillary lymph node
Strain: C3H/HeJ
Tissue: vascular epithelium
Cell Type: endothelialSV40 transformed
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Tumorigenic: Yes
Antigen Expression: H-2 K; VCAM
Age: *****
Gender: male
Comments: The 2H-11 cell line was derived from a solid tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
The line was cloned in 1992 by limiting dilution.
The cells are sensitive to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
2H-11细胞The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), 2H-11细胞and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170
23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612
23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. 2H-11细胞PubMed: 1928299
