BC3细胞
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG2b
ATCC Number： HB-10166™
生长状态： 悬浮生长
数量： 大量
Designations: BC3
Depositors: Fred Hutchinson Cancer Res. Cntr.
Isotype: IgG2b
BC3细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin;BC3细胞 monoclonal antibody; against human CD3 epsilon chain
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with PHA activated human T cells.
BC3细胞Spleen cells were fused with NS-1 mouse myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. BC3细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
