CMT-93细胞
生长状态： 贴壁生长
品系： C57BL/icrf
ATCC Number： CCL-223™
相关**： 其他**
数量： 大量
器官来源： 直肠
细胞形态： 上皮样
是否是肿瘤细胞： 0
物种来源： 小鼠
年限： 19 months
运输方式： 冻存运输
Designations: CMT-93
CMT-93细胞Depositors: LM Franks
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Strain: C57BL/icrf
Organ: rectum
Disease: polyploid carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Antigen Expression: H-2b
GenoType: a(t)
CMT-93细胞Age: 19 months
Gender: male
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. CMT-93细胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22753: Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum.CMT-93细胞 J. Pathol. 124: 35-38, 1978. PubMed: 722371
