AMJ2-C11细胞
年限： 10 weeks old
ATCC Number： CRL-2456™
运输方式： 冻存运输
细胞形态： 单核细胞/巨噬细胞
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
品系： C57BL/6J
生长状态： 悬浮生长，少量贴壁
器官来源： 肺
AMJ2-C11细胞Designations: AMJ2-C11
Depositors: AV Palleroni
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, with some loosely adherent cells
Organism: Mus musculus deposited as mouse
Morphology: macrophage
Source: Organ: lung
Strain: C57BL/6J
Cell Type: macrophage (alveolar); infected with J2 virus
Cellular Products: interleukin-6 (interleukin 6, IL-6)
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Antigen Expression: AMJ2-C11细胞MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 -
Age: 10 weeks old
Gender: female
Comments: AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. [48933]
Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.
Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.
AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).
AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.
The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.
Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma.
The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6.
The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. [48934]
Propagation: AMJ2-C11细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Firmly tap the flask against palm of hand to dislodge any attached cells and transfer along with the floating cells into new flasks.
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48933: Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973
48934: Palleroni AV, et al. AMJ2-C11细胞Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450
