BW5147.G.1.4细胞
生长状态： 悬浮生长
数量： 大量
运输方式： 冻存运输
细胞形态： **样
细胞类型： T**细胞
品系： AKR/J
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： TIB-48™
相关**： **瘤
器官来源： 胸腺
Designations: BW5147.G.1.4
BW5147.G.1.4细胞Depositors: R Hyman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Organ: thymus
Strain: AKR/J
Disease: lymphoma
Cell Type: T lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: This line was derived from BW5147.3 (ATCC TIB-47).
BW5147.G.1.4细胞The cells are resistant to 0.1 mM 6-thioguanine.
Unlike the parental line, these cells are sensitive to HAT.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression: H-2k
Comments: This line was derived from BW5147.3 (ATCC TIB-47).
The cells are resistant to 0.1 mM 6-thioguanine.
Unlike the parental line, these cells are sensitive to HAT.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. BW5147.G.1.4细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
22348: Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329
22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
26346: Goldsby RA, et al. Hybrid cell lines with T-cell characteristics. Nature 267: 707-708, 1977. PubMed: 301614
26347: Hammerling GJ. BW5147.G.1.4细胞T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743-746, 1977. PubMed: 304002
