P3X63Ag8U.1细胞
品系： BALB/c
数量： 大量
细胞形态： **样
细胞类型： B**细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： CRL-1597™
相关**： 多发性骨髓瘤
运输方式： 冻存运输
生长状态： 悬浮生长
Designations: P3X63Ag8U.1
P3X63Ag8U.1细胞Depositors: MD Scharff
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
Permits/Forms: P3X63Ag8U.1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2d
Gender: female
Comments: The cells do not synthesize gamma 1 chains.
They produce but do not secrete kappa chains.
The line was derived from the MOPC 21 - P3X63Ag8 lineage.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: P3X63Ag8U.1细胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 viable cells/ml and maintain below 1 X 10 exp6 cells/ml.
References: 1081: P3X63Ag8U.1细胞Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551
