REC-1细胞
细胞类型： **母细胞
是否是肿瘤细胞： 0
物种来源： 人
年限： 57 years
组织来源： lymph node
数量： 大量
运输方式： 冻存运输
细胞形态： **样
ATCC Number： CRL-3004™
相关**： 其他**
REC-1细胞生长状态： 悬浮生长
Designations: REC-1
Depositors: E. Campo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Characterization Data
Source: Tissue: lymph node
Cell type: lymphoblast
Disease: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
Permits/Forms: REC-1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 2003
Applications: Cyclin D1 is overexpressed.
Oncogene: p53
Antigen Expression: CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC )
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10
D16S539: 11
D5S818: 12,13
D7S820: 10,11
THO1: 9,9.3
TPOX: 8,9
vWA: 17
Cytogenetic Analysis: This is a near-diploid cell line of male origin with a modal chromosome number of 45 and a polyploidy rate of 10%. The karyotype is highly rearranged with most of the cells containing the t(11;14)(q13;q32) common to mantle cell lymphoma. Also, there are approximately 5-6 derivative chromosomes in the karyology that have been described by others. REC-1细胞Note: Cytogenetic information is based on initial seed stock at ATCC .
Age: 57 years
Gender: male
Comments: The human B-cell line Rec-1 was established from a t(11;14)(q13;q32) bearing lymphoma [PubMed: 8142653].
Cyclin D1 is overexpressed. [PubMed: 16434365].
An ongoing level of chromosome rearrangements is observed. [PubMed: 16448697]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Maintain cultures at a cell concentration between 3 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 x 10(6) cells/ml.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Interval: Subculture when cell concentration is between 3x10(5) and 1x10(6) cells/ml.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Preservation: REC-1细胞Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10%
Doubling Time: approximately 38 hours
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
16172765: Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653
16172766: Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242
16172767: Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004
16172768: Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550
16172769: REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm.
