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26CB-1细胞

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  • 产品名称:26CB-1细胞
  • 产品型号:26CB-1
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-04
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简单介绍
26CB-1细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。26CB-1细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

26CB-1细胞

ATCC Number: CRL-1495™

数量: 大量

运输方式: 冻存运输

细胞形态: **样

细胞类型: **母细胞

是否是肿瘤细胞: 0

物种来源: 其他

生长状态: 悬浮生长

Designations: 26CB-1

26CB-1细胞Depositors: LA Falk

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Papio hamadryas

Morphology: lymphoblast


Source: Cell Type: lymphoblast

Permits/Forms: 26CB-1细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: The 26CB-1 cell line was established by co-cultivation of baboon splenic lymphocytes with lethally X-irradiated cells of the 1926 marmoset cell line which carries the HVS transforming virus.

The cells do not produce immunoglobulin.

The cells express a nuclear antigen similar to EBNA and carry Herpesvirus papio.

The cells are E rosette positive.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: 26CB-1细胞Medium Renewal: Every 3 to 5 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and do not permit the cell density to exceed 2 X 10 exp6 cells/ml.

References: 22853: Falk L, et al. Properties of a baboon lymphotropic herpesvirus related to Epstein-Barr virus. Int. J. Cancer 18: 798-807, 1976. PubMed: 186420

22856: Falk LA, et al. Transformation of lymphocytes by Herpesvirus papio. Int. J. Cancer 20: 219-226, 1977. PubMed: 197025

22857: Ohno S, et al. Detection of a nuclear, EBNA-type antigen in apparently EBNA-negative Herpesvirus papio (HVP)-transformed lymphoid lines by the acid-fixed nuclear binding technique. Int. J. Cancer 20: 941-946, 1977. PubMed: 73523

23145: Klein G, et al. Antigen-inducing ability of herpesvirus papio in human and baboon lymphoma lines, compared to Epstein-Barr virus. Intervirology 10: 153-164, 1978.26CB-1细胞 PubMed: 210135

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