产品名称：人肺微血管内皮细胞

人肺微血管内皮细胞(HPMEC)提取于人肺组织，细胞提取纯化之后，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和CD31 (P-CAM)**荧光染色验证，人肺微血管内皮细胞经测试不含有HIV-1、HBV、HCV、支原体、**、酵母和**。细胞可以达到10倍增。

推荐培养基:(ECM, Cat. No. 1001)

人肺微血管内皮细胞Description

The pulmonary microvascular endothelial cells (PMEC) form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. They also have metabolic properties that enable it to carry out certain important nonrespiratory function. The PMEC are among the most important targets of reactive oxygen species elaborated in lung injury [1]. During the course of lung inflammation, neurohumoral mediators and oxidants act on endothelial cells to induce intercellular gaps permissive for transudation of proteinaceous fluid from blood into the interstitium [2]. The increased permeability leads to the hypoxemia associated with ***** respiratory distress syndrome and noncardiogenic pulmonary edema [3].

HPMEC from ScienCell Research Laboratories are isolated from human lung tissue. HPMEC are cryopreserved immediately after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HPMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPMEC are guaranteed to further expand for 10 population doublings at the conditions provided by ScienCell Research Laboratories.
 

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HPMEC in vitro.
 

人肺微血管内皮细胞Product Use

HPMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
 

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
 

人肺微血管内皮细胞Shipping

Dry ice.
 

Reference

[1] Brigham, K. L. (1990) Oxidant stress and ***** respiratory distress syndrome. Eur Respir J Suppl 11: 482s-484s.
[2] Moore T. M., Chetham P. M., Kelly J. J., Stevens T. (1998) Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol. 275(2 Pt 1):L203-22.
[3] Kelly JJ, Moore TM, Babal P, Diwan AH, Stevens T, Thompson WJ. (1998) Pulmonary microvascular and macrovascular endothelial cells: differential regulation of Ca2+ and permeability. Am J Physiol. 274(5 Pt 1):L810-9.