产品名称：人直肠成纤维细胞

收到人直肠成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人直肠成纤维细胞The human rectum is located in the lower gastrointestinal tract and functions as a temporary storage site for fecal material. Rectal fibroblasts are important structural components of the rectum and are implicated in a number of diseases including ulcerative colitis, Crohn’s disease, and rectal cancer. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. The main functions of fibroblasts are to maintain the structural integrity of connective tissue and to aid in tissue repair and remodeling [1, 2]. Studies have shown that functional changes to human rectal fibroblasts (HRecF) can impair wound healing and affect epithelial cell proliferation in ulcerative colitis patients [3]. In Crohn’s disease, HRecF respond to intestinal inflammation by proliferating abnormally, which leads to fibrotic scarring. Primary HRecF can be used to study the pathophysiology of rectal cancer, ulcerative colitis, and Crohn’s disease and to help develop therapies for these diseases.

HRecF from ScienCell Research Laboratories are isolated from human rectum. HRecF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRecF are characterized by immunofluorescence with antibody specific to fibronectin. HRecF are negative for mycoplasma, bacteria, yeast, and fungi. HRecF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HRecF in vitro.

人直肠成纤维细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人直肠成纤维细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人直肠成纤维细胞其它细胞系展示表：HT1080 纤维肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SK-RC-42 肾癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A375 皮肤黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
RCC-krause 肾癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A875 黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Ketr-3 肾癌（瘤株） 品牌ATCC 规格25毫升/株. 货期1-2周
SK-HEL-1 皮肤黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SW 13 肾上腺皮质瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A431 皮肤基底细胞癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
96-C 低转移肺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CEM 白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
95-D 高转移肺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
K562 慢性髓原白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
973 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HPB-ALL T细胞白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A2 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HL-60 白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A549 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Daudi B**细胞瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A549/DDP 肺腺癌顺铂耐药株 品牌ATCC 规格25毫升/株. 货期1-2周
Raji Burkitt**瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Calu-3 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
RAMOS B**细胞瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
GLC-82 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
THP 1 单核细胞型**瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
H1299 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周