产品名称：人结肠微血管内皮细胞

收到人结肠微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人结肠微血管内皮细胞人结肠微血管内皮细胞说明：

Description

Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation [1]. Pharmacological inhibition of NOS in activated intestinal microvascular endothelial cells resulted in a significant increase in leukocytes binding [2]. Gene expression profile study reveals that intestinal endothelial cells express biotinidase, which is involved in biotin recycling [3]. The in vitro culture of these cells enabled scientists to perform systematic analyses of the cytokine profiles with regard to mRNA expression and protein secretion, and to compare such data with cytokine profiles concomitantly displayed by other endothelial cells.

HCoMEC from ScienCell Research Laboratories are isolated from human colonic tissue. HCoMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCoMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HCoMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoMEC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HCoMEC in vitro.

Product Use

HCoMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1]. Ogawa, H., Rafiee, P., Heidemann, J., Fisher, P. J., Johnson, N. A., Otterson, M. F., Kalyanaraman, B., Pritchard, K. A. Jr., Binion, D. G. (2003) Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cells. J Immunol. 170(12):5956-64.

[2]. Binion, D. G., Fu, S., Ramanujam, K. S., Chai, Y. C., Dweik, R. A., Drazba, J. A., Wade, J. G., Ziats, N. P., Erzurum, S. C. and Wilson, K. T. (1998) iNOS xpression in human intestinal microvascular endothelial cells inhibits leukocyte adhesion. Am. J. Physiol. 275:G592-603.

[3]. Chi, J-T, Chang, H. Y., Haraldsen, G., Jahnsen, F. L., Troyanskaya, O. G., Chang, D. S., Wang, Z., Rockson, S. G., van de Rijn, M., Botstein, D. and Brown, P. O. (2003) Endothelial cell diversity revealed by global expression profiling. PNAS 100(19):10623-10628.

人结肠微血管内皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人结肠微血管内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人结肠微血管内皮细胞其它细胞系展示表：45.6.TG1.7 骨髓瘤 品牌ATCC 规格25毫升/株. 货期1-2周
S180 腹水瘤 品牌ATCC 规格25毫升/株. 货期1-2周
P3-X63-Ag8 骨髓瘤 品牌ATCC 规格25毫升/株. 货期1-2周
B16 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
J774A.1 单核细胞－巨噬细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C127 乳腺肿瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RAW264.7 单核细胞－巨噬细胞 品牌ATCC 规格25毫升/株. 货期1-2周
F9 胚胎瘤 品牌ATCC 规格25毫升/株. 货期1-2周
NG108-15 小鼠神经细胞瘤×大鼠神经胶质细胞杂交细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C6 胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RH－35 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
SHZ－88 乳腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
CBRH－7919 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
PC-12 肾上腺嗜铬细胞瘤 品牌ATCC 规格25毫升/株. 货期1-2周
A431 表皮癌 品牌ATCC 规格25毫升/株. 货期1-2周
QGY-7701 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
A673 横纹肌癌 品牌ATCC 规格25毫升/株. 货期1-2周
QGY-7703 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
Tca-8113 舌鳞癌 品牌ATCC 规格25毫升/株. 货期1-2周
SMMC-7721 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
Acc-2 涎腺腺样囊性癌 品牌ATCC 规格25毫升/株. 货期1-2周
786-O 肾透明细胞腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
Acc-3 涎腺腺样囊性癌 品牌ATCC 规格25毫升/株. 货期1-2周
PC-3 前列腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
KB 口腔表皮样癌 品牌ATCC 规格25毫升/株. 货期1-2周
T-24 膀胱变移细胞癌 品牌ATCC 规格25毫升/株. 货期1-2周
HEp-2 喉表皮样癌 品牌ATCC 规格25毫升/株. 货期1-2周
SCaBER 膀胱鳞癌 品牌ATCC 规格25毫升/株. 货期1-2周
CNE 鼻咽癌 品牌ATCC 规格25毫升/株. 货期1-2周
Ho-8910 卵巢癌 品牌ATCC 规格25毫升/株. 货期1-2周