产品名称：人肠道成纤维细胞

收到人肠道成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人肠道成纤维细胞人肠道成纤维细胞说明：

Description

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowl stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion [3].

HIF from ScienCell Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HIF in vitro.

Product Use

HIF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.

[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.

[3] Luna, J., Masamunt, MC., Rickmann, M., Mora, R., Espana, C., Delgado, S., Llach, J., Vaquero, E., Sans, M. (2011) Rocotrienols have potent antifibrogenic effects in human intestinal fibroblasts. Inflamm Bowel Dis. 17(3):732-41

人肠道成纤维细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人肠道成纤维细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人肠道成纤维细胞其它细胞系展示表：TT 髓性甲状腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
L1 卵巢癌 品牌ATCC 规格25毫升/株. 货期1-2周
Bcap-37 乳腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HeLa 宫颈癌 品牌ATCC 规格25毫升/株. 货期1-2周
ZR-75-30 乳腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HeLa229 宫颈癌 品牌ATCC 规格25毫升/株. 货期1-2周
MCF-7 乳腺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
KB-C1.5 宫颈癌 品牌ATCC 规格25毫升/株. 货期1-2周
SK-BR-3 乳腺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HCC **高分化鳞癌 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-435S 乳腺管癌 品牌ATCC 规格25毫升/株. 货期1-2周
JAR 胎盘绒毛癌 品牌ATCC 规格25毫升/株. 货期1-2周
T47D 乳腺管癌 品牌ATCC 规格25毫升/株. 货期1-2周
RD 恶性胚胎横纹肌瘤 品牌ATCC 规格25毫升/株. 货期1-2周
ybL-100 乳腺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BT-325 神经胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SMC-1 恶性胸膜间皮瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SK-N-SH 神经母细胞瘤 品牌ATCC 规格25毫升/株. 货期1-2周
A2 肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
SHG-44 胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
95-D 高转移肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
A375 恶性黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
Calu-3 肺腺癌 （胸膜渗出液） 品牌ATCC 规格25毫升/株. 货期1-2周
Bowes Melanoma 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
LTEP-a-2 肺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
MM96L 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SH-77 小细胞肺癌 品牌ATCC 规格25毫升/株. 货期1-2周