产品名称：人食道微血管内皮细胞

收到人食道微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人食道微血管内皮细胞Description

Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that esophageal microvascular endothelial cells exhibit a strong inflammatory immune response to LPS, cytokines, and pH exposure, thus playing a critical regulatory role in esophageal inflammation [1]. When exposed to acidic pH, HEMEC express some proteins, such as VCAM-1, but not others, such as ICAM-1, which is speculated to selectively recruit leukocytes to the esophagus [2]. This could play a role in the cellular response triggered by gastroesophageal reflux disease. In vitro cultures of HEMEC have enabled scientists to observe leukocyte binding, upregulation of cell adhesion molecules, and other cellular responses to LPS, cytokines and pH exposure that indicate that HEMECs are intrinsically different from lower gastrointestinal endothelial cells in regards to an inflammatory immune response [3].

HEMEC from ScienCell Research Laboratories are isolated from human esophageal tissue. HEMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HEMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEMEC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HEMEC in vitro.

Product Use

HEMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1], [3]. Rafiee, P., Ogawa, H., Heidemeann, J., Li, MS., Aslam, M., Lamirand, TH., Risher, PJ., Graewin, SJ., Dwinell, MB., Johnson, CP., Shaker, R., Binion, DG. (2003) Isolation and characterization of human esophageal microvascular endothelial cells: mechanisms of inflammatory activation. Am J Physiol Gastroinest Liver Physiol. 285(6): G1277-92.

[2]. Rafiee, P., Theriot, ME,. Nelson, VM., Heidemann, J., Kanaa, Y., Horowitz, SA., Roqaczewski, A., Johnson, CP., Ali, I., Shaker, R., Binion, DG. (2006) Human esophageal microvascular endothelial cells respond to acidic pH stree by PI3K/AKT and p38 MAPK-regulated induction of Hsp70 and Hsp27. Am J Physiol Cell Physiol. 291(5): C931-45.

人食道微血管内皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人食道微血管内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人食道微血管内皮细胞其它细胞系展示表：yb-hxbz-089 人胚胎胰腺组织来源细胞 CCC-HPE-2 规格25毫升/株.
yb-hxbz-090 人胰腺腺泡上皮癌 HPAC 规格25毫升/株.
yb-hxbz-091 人胰腺腺癌细胞 Panc 10.05 规格25毫升/株.
yb-hxbz-092 人肝癌细胞 HepG-2 规格25毫升/株.
yb-hxbz-093 人肝癌细胞 HepG2.2.1.5 规格25毫升/株.
yb-hxbz-094 人肝癌细胞 SMMC-7721 规格25毫升/株.
yb-hxbz-095 人肝癌细胞 QGY-7701 规格25毫升/株.
yb-hxbz-096 人肝癌细胞 QGY-7703 规格25毫升/株.
yb-hxbz-097 人肝癌细胞 Bel-7402 规格25毫升/株.
yb-hxbz-098 人肝癌细胞 Bel-7404 规格25毫升/株.
yb-hxbz-099 人肝癌细胞 Bel-7405 规格25毫升/株.
yb-hxbz-100 人肝癌细胞 Hep3B2.1-7 规格25毫升/株.
yb-hxbz-101 人肝癌细胞 SK-HEP-1 规格25毫升/株.
yb-hxbz-102 人肝癌亚力山大细胞 HCCC-9810 规格25毫升/株.
yb-hxbz-103 人胆囊癌细胞 GBC-SD 规格25毫升/株.
yb-hxbz-104 正常人肝细胞 L-02 规格25毫升/株.
yb-hxbz-105 人胚肝细胞正常 QSG-7701 规格25毫升/株.
yb-hxbz-106 人肝细胞正常 HL-7702 规格25毫升/株.
yb-hxbz-107 CCL13张氏肝细胞正常 Chang liver 规格25毫升/株.
yb-hxbz-108 人肝星形细胞正常 LX-2 规格25毫升/株.
yb-hxbz-109 人肝内胆管上皮细胞 HIBEpiC 规格25毫升/株.
yb-hxbz-110 人高转移肝癌细胞 LM3 规格25毫升/株.
yb-hxbz-111 人肝癌细胞系 LM-6 规格25毫升/株.
yb-hxbz-112 人低转移肝癌细胞 MHCC97-L 规格25毫升/株.
yb-hxbz-113 人肝癌细胞 HepG2/2-15 规格25毫升/株.
yb-hxbz-114 人肝癌细胞 9204 规格25毫升/株.
yb-hxbz-115 人肝癌细胞 HuH-7 规格25毫升/株.
yb-hxbz-116 人肝癌细胞 FL62891 规格25毫升/株.