产品名称：人气管成纤维细胞

收到人气管成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人气管成纤维细胞Fibroblasts are mesenchymal cells which perform many vital functions during development and *****hood. Fibroblasts are responsible for the majority of the extracellular matrix synthesis in connective tissue and play major roles in wound healing. In the trachea, fibroblasts and epithelial cells interact in order to respond to tissue injury, bacterial contamination, and other environmental factors [1]. Studies in vitro and in vivo show that tracheal fibroblasts (TrF) can promote tracheal epithelial regeneration by influencing epithelial cell proliferation and differentiation [2, 3]. Abnormal proliferation of Human TrF (HTrF) may lead to the development of airway diseases, such as asthma [1]. HTrF are a useful model for elucidating the mechanisms of wound healing and may provide novel insights for tissue regeneration.

HTrF from ScienCell Research Laboratories are isolated from human trachea. HTrF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTrF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HTrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTrF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Catalog No. 3430

Country of Manufacture United States

Product Code HTrF

Size/Quantity >5 x 105 cells in 1 ml volume

Product Use HTrF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping Info Dry ice.

References [1] Evan M, Fanucchi M, Van Winkle L, Baker G, Murphy A, Nishio S, Sannes P, Plopper C. (2002) "Fibroblast growth factor-2 during postnatal development of tracheal basement membrane zone." Am J Physiol Lung Cell Mol Physiol. 283(6): L1263-1270.

[2] Kobayashi K, Nomoto Y, Suzuki T, Tada Y, Miyake M, Hazama A, Kanemaru S, Nakamura T, Omori K. (2006) "Effect of fibroblasts on tracheal epithelial regeneration in vitro." Tissue Eng. 12(9): 2619-2628.

[3] Goto Y, Noguchi Y, Nomura A, Sakamoto T, Ishii Y, Bitoh S, Picton C, Fujita Y, Watanabe T, Hasegawa S, Uchida Y. (2002) "In vitro reconstitution of the tracheal epithelium." Am J Respir Cell Mol Biol. 20(2): 312-3

人气管成纤维细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人气管成纤维细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人气管成纤维细胞其它细胞系展示表：yb-hxbz-117 人胆管细胞型肝癌细胞 HCCC-9810 规格25毫升/株.
yb-hxbz-118 人肝癌细胞 HCC-9724 规格25毫升/株.
yb-hxbz-119 人肝癌细胞 HHCC 规格25毫升/株.
yb-hxbz-120 人肝癌细胞系 Hep-3B 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-hxbz-121 表达HBV病毒的人肝细胞 HepG2.2.15 规格25毫升/株.
yb-hxbz-122 人肝癌细胞 H7402 规格25毫升/株.
yb-hxbz-123 人胚肝二倍体细胞 CCC-HEL-1 规格25毫升/株.
yb-hxbz-124 人肝细胞 FL62891 规格25毫升/株.
yb-hxbz-125 人感染HCV肝癌细胞 HuH7-HCV 规格25毫升/株.
yb-hxbz-126 永生化鼻咽上皮细胞系 NP69-SV40T 规格25毫升/株.
yb-hxbz-127 人鼻咽癌母系细胞 CNE-2Z 规格25毫升/株.
yb-hxbz-128 人鼻咽癌细胞 CNE 规格25毫升/株.
yb-hxbz-129 人鼻咽癌细胞（高分化） CNE1 规格25毫升/株.
yb-hxbz-130 人鼻咽癌细胞（低分化） CNE2 规格25毫升/株.
yb-hxbz-131 人鼻咽癌细胞 HNE1 规格25毫升/株.
yb-hxbz-132 人鼻咽癌细胞 HNE2 规格25毫升/株.
yb-hxbz-133 人鼻咽癌细胞 HNE3 规格25毫升/株.
yb-hxbz-134 人鼻咽癌细胞 HONE1 规格25毫升/株.
yb-hxbz-135 转基因鼻咽癌细胞系 CNE1-lmp\l 规格25毫升/株.
yb-hxbz-136 低分化鼻咽癌细胞系 SUNE-1 规格25毫升/株.
yb-hxbz-137 鼻咽癌细胞系 SUNE-1亚株-5-8F 规格25毫升/株.
yb-hxbz-138 5-8转基因鼻咽癌细胞系 F2-2 规格25毫升/株.
yb-hxbz-139 人鼻咽癌细胞系 SUNE-1亚株6-10B 规格25毫升/株.
yb-hxbz-140 人鼻咽癌细胞系 SUNE-1亚株13-9B 规格25毫升/株.
yb-hxbz-141 人鼻咽癌细胞系 HNE2-LMP1/2 规格25毫升/株.