产品名称：人心包成纤维细胞

收到人心包成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人心包成纤维细胞Description

Pericardial Fibroblasts are the principal cell type of the serous pericardium. They help to form the strong connective tissue that allows the pericardium to serve as a protective sac for the heart. Pericardial fibroblasts are exposed to a dynamic mechanical environment, being attached to the diaphragm, which influences the structure integrity of both healthy and healing tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1] and there is good evidence that fibroblasts in different parts of the body are intrinsically different [2]. Fibroblasts have been extensively used for a wide range of cellular and molecular studies because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection.

HPcF from ScienCell Research Laboratories are isolated from the human heart. HPcF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescent method with antibody to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPcF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HPcF in vitro.

Product Use

HPcF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.

[2] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137