产品名称：人心脏成纤维细胞--**

收到人心脏成纤维细胞--**时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人心脏成纤维细胞--**Description

The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction [1], cardiac fibrosis, and is often complicated by cardiac hypertrophy [2]. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression [3]. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.

HCF from ScienCell Research Laboratories are isolated from the human heart. HCF-a are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCF-a are characterized by immunofluorescent method with antibody to fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-a are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. No. 2331) for the culturing of HCF-a in vitro

Product Use

HCF-a are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9.

[2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54.

[3] Burgess, M. L., Terracio, L, Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87.

人心脏成纤维细胞--**传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人心脏成纤维细胞--**倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人心脏成纤维细胞--**其它细胞系展示表：yb1662 AEpiCM 肺泡上皮细胞培养基 Alveolar Epithelial Cell Medium 500 ml
yb1663 KM 角质细胞培养基 Keratinocyte Medium 500 ml
yb1664 干细胞检测试剂盒
yb1665 HGL PCR 人胚层检测试剂盒 Human Germ Layer Detection Kit 50 reactions
yb1666 HSC PCR 人干细胞多能性检测试剂盒 Human Stem Cell Pluripotency Detection Kit 50 reactions
yb1667 ALP 碱性磷酸酶活性测定试剂盒 Alkaline Phosphatase Activity Assay 500 tests
yb1668 ALPr 碱性磷酸酶染色检测（红色） Alkaline Phosphatase Staining Assay (Red) 500 tests
yb1669
yb1670 Cell Transfection Kits
yb1671 EndoFectagen. 内皮细胞转染试剂盒 Endothelial Cell Transfection Kit 250 Transfections
yb1672 FibroFectagen. 成纤维细胞转染试剂 Fibroblast Transfection Kit 200 Transfections
yb1673 EpiFectagen. 上皮细胞转染试剂盒 Epithelial Cell Transfection Kit 100 Transfections
yb1674 MesenFectagen. 间充质干细胞转染试剂盒 Mesenchymal Stem Cell Transfection Kit 250 Transfections
yb1675 AstroFectagen. 星形胶质细胞转染试剂盒   Astrocyte Transfection Kit 250 Transfections
yb1676 SMCFectagen. 平滑肌细胞转染试剂盒 Smooth Muscle Cell Transfection Kit 250 Transfections
yb1677 KeratoFectagen. 角质细胞转染试剂盒 Keratinocyte Transfection Kit 200 Transfections
yb1678 EpiFectagen II. 上皮细胞转染试剂盒   Epithelial Cell Transfection Kit 250 Transfections
yb1679
yb1680 Cell Based Assay
yb1681 血管生成检测试剂盒
yb1682 IVTFA 体外管腔形成分析试剂盒 In Vitro Tube Fomation Assay (Angiogenesis Assay) 50 tests/96 well plate
yb1683 细胞粘附试剂盒