- 入驻时间： 2015-11-06
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- Email：shybio@126.com
收到人结膜上皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。
人结膜上皮细胞Description
The conjunctival epithelium is a stratified, non-keratinized epithelium. It can be distinguished by the corneal epithelium by the expression of different cytokeratins, mucins and by the presence of glycocalix [1]. Decrease or loss of mucin/glycocalix production generates squamous metaplasia, which may lead to dry eye and ocular surface diseases [2]. Human conjunctival epithelial cells represent potential target cells for mast cell mediators. They respond to histamine by generating inositol phosphates, mobilizing intracellular calcium, and then secreting proinflammatory cytokines like interleukin-6 and interleukin-8 [3].
HConEC from ScienCell Research Laboratories are isolated from human conjunctiva. HConEC are cryopreserved from passage one culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HConEC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HConEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConEC are guaranteed to further expand for 10 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Corneal Epithelial Cell Medium (CEpiCM, Cat. No. 6511) for the culturing of HConEC in vitro.
Product Use
HConEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1]. Meller D., Pires R. T. and Tseng S. C. (2002) Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br.J.Ophthalmol. 86:463-471.
[2]. Paladino G., Marino C., La Terra Mule s., Civiale C., Rusciano D. and Enea V (2004) Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva. Tissue Cell 36:323-332.
[3]. John M. Yanni, Lori K. Weimer, Najam A. Sharif, Shou X. Xu, Daniel A. Gamache, Joan M. Spellman, (1999) Inhibition of Histamine-Induced Human Conjunctival Epithelial Cell Responses by Ocular. Allergy Drugs. Arch Ophthalmol. 117:643-647.
人结膜上皮细胞传代方法：
收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人结膜上皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法： 冻存液：基础培养基+5%DMSO+20%FBS
储存：液氮储存
人结膜上皮细胞其它细胞系展示表：yb-1101 DU145(前列腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周 Mo-MuLV/3T3小鼠Mo-MuLv感染的3T3细胞
yb-1102 Lncap(前列腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1103 PC-3(前列腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1104 MOLT-4(急性**母细胞白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1105 5637(膀胱癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1106 Raji(Burkitt's **瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1107 SCaBER(膀胱鳞癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1108 NAMALWA(Burkitt's **瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1109 T24(膀胱移行细胞癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1110 HuT78(T**细胞白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1111 SW-13(肾上腺皮质腺癌细胞 ). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1112 SW-13(肾上腺皮质腺癌细胞 ). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1113 Dami(巨核细胞白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1114 OS-RC-2(肾癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1115 MEG-01. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1116 786-O [786-0](肾透明细胞腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1117 786-O [786-0](肾透明细胞腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1118 K-562(慢性髓原白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1119 K-562(慢性髓原白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1120 Hce-8693(盲肠腺癌细胞(未分化)). 品牌ATCC 规格25毫升/株. 货期1-2周
