产品名称：人脂肪微血管内皮细胞

收到人脂肪微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人脂肪微血管内皮细胞Description

Microvascular endothelial cells line blood vessels and contribute to many biological processes such as angiogenesis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells are diverse and have specific cellular characteristics and functions depending on the organ/tissue in which they are located. Adipose tissue is unique because it has the capacity to continually grow throughout ***** life. Thus, it has a high level of angiogenesis to provide the extensive vascularization required for adipose tissue [1]. Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes [2]. At the same time, microvascular endothelial cell growth is stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationship between microvascular endothelial cells and preadipocytes during tissue development [3].

HAMEC from ScienCell Research Laboratories are isolated from human adipose tissue. HAMEC are cryopreserved and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM) and by uptake of DiI-Ac-LDL. HAMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HAMEC.

Product Use

HAMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Crandall D.L., Hausman G.J., Kral J.G. (1997) A review of the microcirculation of adipose tissue: anatomic, metabolic, and angiogenic perspectives. Microcirculation 4(2):211-32

[2] Hutley, L. J., Herington, A. C., Shurety, W., Cheung, C., Vesey, D. A., Cameron, D. P., Prins, J. B. (2000) Human adipose tissue endothelial cells promote preadipocyte proliferation. American Journal of Physiology: Endocrinology and Metabolism. 281(5):E1037-E1044

[3] Hausman, G. J., Richardson, R. L. (2004) Adipose tissue angiogenesis. Journal of Animal Science. 82(3): 925-934