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收到HCerMEC人**颈微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。
HCerMEC人**颈微血管内皮细胞The cervix is the lower portion of the uterus and functions to promote fertility and protect the female reproductive tract and fetus from potential pathogens. In addition, the cervix plays a critical role in childbirth by effacing and dilating to allow the baby to pass out of the uterus [1]. Vascular endothelial cells lining the blood vessels actively participate in many vital biological processes. Cervical Microvascular Endothelial Cells (CerMEC) limit the passage of cellular and soluble substances between the blood vessels and the surrounding tissues [2]. CerMEC also play critical roles in angiogenesis, vasculogenesis, and the inflammatory response [2]. CerMEC angiogenesis has been shown to contribute to the progression of cervical cancer [3]. Recent studies suggest that CerMEC may even initiate signaling events which directly promote tumor growth [3]. Cultured human CerMEC (HCerMEC) are a useful model to elucidate the mechanisms of normal and pathological angiogenesis and develop therapeutic treatments for cervical cancer.
HCerMEC from ScienCell Research Laboratories are isolated from human cervix. HCerMEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCerMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HCerMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCerMEC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HCerMEC in vitro.
HCerMEC人**颈微血管内皮细胞传代方法：
收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，HCerMEC人**颈微血管内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法： 冻存液：基础培养基+5%DMSO+20%FBS
储存：液氮储存
HCerMEC人**颈微血管内皮细胞其它细胞系展示表：yb-2196. GC-2spd(ts)（小鼠精母细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-249. NCL-H548（人胰腺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-138. Hs 683（人脑视神经胶质瘤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-7002. Hs 1.Tes（正常人睾丸细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-7131. Hs 181.Tes（正常人睾丸细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
24858. Y567 [VTT C-79094]. 品牌ATCC 规格25毫升/株. 货期1-2周
24683. [X464-20C]. 品牌ATCC 规格25毫升/株. 货期1-2周
26292. NCYC 234 [IPAZSC 148]. 品牌ATCC 规格25毫升/株. 货期1-2周
26785. Y-99. 品牌ATCC 规格25毫升/株. 货期1-2周
55669. STJ.EPI.H7. 品牌ATCC 规格25毫升/株. 货期1-2周
15834. [CIP 104786]. 品牌ATCC 规格25毫升/株. 货期1-2周
11325. [CIP 104328; IAM 13570; ICMP 5794; NCPPB 2991]. 品牌ATCC 规格25毫升/株. 货期1-2周
25818. ICPB TR7 [C-1; ICMP 8639; NCPPB 2626]. 品牌ATCC 规格25毫升/株. 货期1-2周
43056. R1000. 品牌ATCC 规格25毫升/株. 货期1-2周
43057. A4. 品牌ATCC 规格25毫升/株. 货期1-2周
31798. MSU-1. 品牌ATCC 规格25毫升/株. 货期1-2周
31799. MSU-2. 品牌ATCC 规格25毫升/株. 货期1-2周
22064. NRRL 2543 [LSHTM BB325]. 品牌ATCC 规格25毫升/株. 货期1-2周
32189. R3. 品牌ATCC 规格25毫升/株. 货期1-2周
200757. M-37. 品牌ATCC 规格25毫升/株. 货期1-2周
293T 人胚肾T细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CCC-ESF-1 人胚胎皮肤成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CCC-HPF-1 人胚肺二倍体细胞 品牌ATCC 规格25毫升/株. 货期1-2周
DMF7 双位点HC-KIT受体细胞株 品牌ATCC 规格25毫升/株. 货期1-2周
FC33 ASP2人胚胎肾细胞转化细胞 品牌ATCC 规格25毫升/株. 货期1-2周
FIP293 FIP293(来源于HEK293) 品牌ATCC 规格25毫升/株. 货期1-2周
HEK-293 人胚肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HFF 人前皮肤成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
