产品名称：人卵巢微血管内皮细胞

收到人卵巢微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人卵巢微血管内皮细胞Description

Angiogenesis is critical for many physiological processes including organ development and tissue repair [1]. In human ovaries, angiogenesis is known to be associated with the development of follicles and the formation of the corpus luteum. A complex vascular network is formed within the thecal cell layer during follicular growth, and rapid neovascularization occurs toward the granulosa cell layer after ovulation [2]. Endothelial cells from different organs, as well as from large and small vessels of the same organ, have specialized properties [3]. The endothelial cells prepared from the specific microvasculature of the ovarian follicle are an ideal in vitro model for the study of the angiogenesis and the physiology of the female reproductive system.

HOMEC from ScienCell Research Laboratories are isolated from human ovarian tissue. HOMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HOMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HOMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOMEC are guaranteed to further expand for 10 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HOMEC in vitro.

Product Use

HOMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1]. Scott, P.A.E. and Bicknell, R. (1993) The isolation and culture of microvascular endothelium. J. Cell Sci., 105, 269-273.

[2]. Naoko Otani, Sawako Minami, Mareo Yamoto, Toshihiko Shikone, Hisako Otani, Rika Nishiyama, Tsutomu Otani and Ryosuke Nakano (1999) The Vascular Endothelial Growth Factor/fms-Like Tyrosine Kinase System in Human Ovary during the Menstrual Cycle and Early Pregnancy. J. Clinical Endocrinology & Metabolism 84(10)3845-3851.

[3]. Gerritsen, M. E. (1987) Function heterogeneity of vascular endothelial cells. Biochem. Pharmacol. 36:2701-2711.

人卵巢微血管内皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人卵巢微血管内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人卵巢微血管内皮细胞其它细胞系展示表：yb-1304 RAW 264.7(单核巨噬细胞白血病). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1305 P19(畸胎癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1306 C6(胶质瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1307 RBL-2H3(白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1308 SiHa(**颈癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1309 MG-63(骨肉瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1310 HEC-1-B(**内膜腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1311 SW1353(骨肉瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1312 ES-2(卵巢透明细胞癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1313 Saos-2(成骨肉瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1314 MDA-MB-468(乳腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1315 SK-MES-1(肺鳞癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1316 MDA-MB-435S(乳腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1317 NCI-H661(大细胞肺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1318 ZR-75-1(乳腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1319 NCI-H292(肺癌细胞（**结转移）). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1320 Hs 578T(乳腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1321 U-937(组织细胞**瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1322 CFPAC-1(胰腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1323 A3(**细胞白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1324 PANC-1(胰腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1325 Jurkat,Clone E6-1(T **细胞白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1326 SK-HEP-1(肝癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1327 HL-60(急性粒细胞白血病细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1328 HCCLM3(肝癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周