产品名称：小鼠骨髓单核细胞

收到小鼠骨髓单核细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠骨髓单核细胞Bone Marrow Mononuclear Cells (BMMC) are located in the bone marrow and have regenerative capabilities. Primary BMMC are a heterogeneous population of single nucleus cells which include hematopoietic stem cells, hematopoietic progenitor cells, immature monocytes, and immature lymphocytes [1]. Recent studies suggest that BMMC are suitable as a cell therapy to treat a variety of diseases such as cardiovascular disease, type 1 diabetes, and ischemic stroke [2, 3, 4]. BMMC may also be used for bone tissue engineering [1]. Primary mouse BMMC (MBMMC) can be utilized to further study BMMC as a potential cell therapy.

MBMMC from ScienCell Research Laboratories are isolated from healthy ***** CD-1 mouse bone marrow. MBMMC are depleted of erythrocytes and bone marrow macrophages, cryopreserved immediately after isolation, and delivered frozen. MBMMC are a mixed population of cells that include hematopoietic stem cells and progenitor cells, monocytes, and lymphocytes. Each vial contains at least 10 million cells in 1 ml volume. MBMMC are quality control tested for viability. MBMMC are negative for mycoplasma, bacteria, yeast, and fungi. MBMMC can be maintained for a short period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Catalog No. M1930

Country of Manufacture United States

Product Code MBMMC

Size/Quantity 10 million cells in 1 ml volume

Product Use MBMMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping Info Dry ice.

References [1] Henrich D, Verboket R, Schaible A, Kontradowitz K, Oppermann E, Brune J, Nau C, Meier S, Bonig H, Marzi I, Seebach C. (2015) "Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro." Biomed Res Int. 10: 1-12.

[2] Savitz S, Misra V, Kasam M, Juneja H., Cox C, Alderman S, et al. (2011). "Intravenous autologous bone marrow mononuclear cells for ischemic stroke." Ann Neurol. 70:59-69.

[3]Fotino C, Ricordi C, Lauriola V, Alejandro R, Pileggi A. (2010) "Bone marrow-derived stem cell transplantation for the treatment of insulin-dependent diabetes." Rev Diabet Stud. 7(2): 144-157.

[4] Arnous S, Mozid A, Martin J, Mathur A. (2012) "Bone marrow mononuclear cells and acute myocardial infarction

小鼠骨髓单核细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠骨髓单核细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠骨髓单核细胞其它细胞系展示表：M2 待查 品牌ATCC 规格25毫升/株. 货期1-2周
MCF7 人乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MCF7B 人乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-157 人乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-231 人乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-435s 人乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-453 人乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MG-63 人骨肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MOLT-4 人**细胞白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
NCI-H157 人非小细胞肺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
NCI-H209 人小细胞肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
NCI-H446 人小细胞肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
NTERA-2 人恶性多发性畸胎瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PA-1 人卵巢畸胎瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PANC-1 人胰腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PC-3 人前列腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
RAJI 黑人Burkitt**瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RAMOS 人B**细胞瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RAMOS(RA.1) 人B**细胞瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RPMI-8226 人多发骨髓瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周