产品名称：小鼠肝巨噬细胞

收到小鼠肝巨噬细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠肝巨噬细胞Description

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Mouse Hepatic Macrophages (MHMa), which are also known as Kupffer cells, reside within the lumen of liver sinusoids. MHMa protect the liver by responding to pathogens and metastatic cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the portal vein and hepatic artery [1]. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation [2-4]. MHMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.

MHMa from ScienCell Research Laboratories are isolated from postnatal day 2 mouse liver. MHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume. MHMa are characterized by immunofluorescence with antibody to F4/80. MHMa are negative for mycoplasma, bacteria, yeast, and fungi. MHMa are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, RHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing MHMa in vitro

Product Use

MHMa are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Liaskou E, Wilson DV, Oo YH. (2012) "Innate immune cells in liver inflammation."Mediators Inflamm. 2012: 949157.

[2] Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, Wouters K, Lutjohann D, Gijbels MJ, Febbraio M, Binder CJ, Hofker MH, Shiri-Sverdlov R. (2012) "Internalization of modified lipids by CD36 and SR-A leads to hepatic inflammation and lysosomal cholesterol storage in Kupffer cells."PLoS One. 7: e34378.

[3] Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA. (2006) "Kupffer cell-dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse." Proc Natl Acad Sci U S A. 103: 4598-603.

[4] Seki E, de Minicis S, Inokuchi S, Taura K, Miyai K, van Rooijen N, Schwabe RF, Brenner DA. (2009) "CCR2 promotes hepatic fibrosis in mice." Hepatology. 50: 185-97

小鼠肝巨噬细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠肝巨噬细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠肝巨噬细胞其它细胞系展示表：OS-732 骨肉瘤细胞（瘤株） 品牌ATCC 规格25毫升/株. 货期1-2周
Panc 10.05 胰腺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A-204 横纹肌肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SW1990 胰腺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HT1080 纤维肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SK-RC-42 肾癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A375 皮肤黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
RCC-krause 肾癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A875 黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Ketr-3 肾癌（瘤株） 品牌ATCC 规格25毫升/株. 货期1-2周
SK-HEL-1 皮肤黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SW 13 肾上腺皮质瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A431 皮肤基底细胞癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
96-C 低转移肺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CEM 白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
95-D 高转移肺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
K562 慢性髓原白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
973 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HPB-ALL T细胞白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A2 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HL-60 白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A549 肺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Daudi B**细胞瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周