产品名称：小鼠肝星状细胞

收到小鼠肝星状细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠肝星状细胞Description

Hepatic stellate cells (HSteC) are pericytes found in the perisinusoidal space and exhibit myofibroblast-like or lipocyte phenotypes. They participate in the homeostasis, repair, and regeneration of liver extracellular matrix, and control retinol metabolism, storage, and release. Following liver injury, HSteC proliferate, transform into myofibroblast-like cells, and produce type I collagen in the fibrotic liver. HSteC have been implicated as a regulator of hepatic microcirculation via cell contraction in the pathogenesis of intrahepatic portal hypertension [1]. HSteC also have voltage-activated calcium channels, express the low affinity nerve growth factor receptor p75, and respond to nerve growth factor stimulation by undergoing apoptosis [2, 3]. Understanding the molecular regulation of HSteC activation may lead to the development of new therapies for treatment of hepatic fibrosis and chronic liver disease.

MHSteC from ScienCell Research Laboratories are isolated from postnatal day 2 mouse liver. MHSteC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MHSteC are characterized by immunofluorescence with antibodies specific to desmin and α-actin. MHSteC are negative for mycoplasma, bacteria, yeast, and fungi. MHSteC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Stellate Cell Medium (SteCM, Cat. #5301) for culturing MHSteC in vitro.

Product Use

MHSteC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Reynaert H, Thompson MG, Thomas T, Geerts A. (2002) "Hepatic stellate cells: role in microcirculation and pathophysiology of portal hypertension." Gut 50: 571-81.

[2] Oide H, Tateyama M, Wang XE, Hirose M, Itatsu T, Watanabe S, Ochi R, Sato N. (1999) "Activated stellate (Ito) cells possess voltage-activated calcium current." Biochim Biophys Acta. 1418: 158-64.

[3] Trim N, Morgan S, Evans M, Issa R, Fine D, Afford S, Wilkins B, Iredale J. (2000) "Hepatic stellate cells express the low affinity nerve growth factor receptor p75 and undergo apoptosis in response to nerve growth factor stimulation." Am J Pathol. 156: 1235-43.