产品名称：小鼠肾系膜细胞

收到小鼠肾系膜细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠肾系膜细胞Description

Renal mesangial cells are perivascular cells located within the central portion of the glomerular tuft between capillary loops, constituting 30-40% of the total glomerular cell population [1]. They regulate the intraglomerular capillary flow and ultrafiltration surface via mesangial cell contraction and release of growth factors and vasoactive agents [2]. By pinocytosis and phagocytosis, mesangial cells remove local accumulation of macromolecules in the mesangial space [3]. Mesangial cells also synthesize, assemble and control turnover of the mesangial matrix. Overproduction of mesangial cells has been observed in various glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy [4]. Such critical involvements suggest renal mesangial cells are an ideal model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.

MRMC from ScienCell Research Laboratories are isolated from postnatal day 2 mouse kidney. MRMC are cryopreserved at passage 1 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MRMC are characterized by immunofluorescence with antibodies specific to fibronectin, Thy-1, and smooth muscle actin. MRMC are negative for mycoplasma, bacteria, yeast, and fungi. MRMC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesangial Cell Medium (MCM, Cat. No. 4201) for the culturing of MRMC in vitro.

Product Use

MRMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Olivetti G, Anversa P, Rigamonti W, Vitali-Mazza L, Loud AV. (1977) "Morphometry of the renal corpuscle during normal postnatal growth and compensatory hypertrophy. A light microscope study." J Cell Biol. 75: 573-85.

[2] Gruden G, Thomas S, Burt D, Zhou W, Chusney G, Gnudi L, Viberti G. (1999) “Interaction of angiotensin II and mechanical stretch on vascular endothelial growth factor production by human mesangial cells.” J Am Soc Nephrol. 10: 730-7.

[3] Gómez-Guerrero C, Suzuki Y, Egido J. (2002) “The identification of IgA receptors in human mesangial cells: in the search for "Eldorado".” Kidney Int. 62: 715-7.

[4] Abboud HE. (2012) “Mesangial cell biology.” Exp Cell Res. 318: 979-85

小鼠肾系膜细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠肾系膜细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠肾系膜细胞其它细胞系展示表：T47D 乳腺导管癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF763 脑瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
ZR-75-1 乳腺导管癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF767 脑瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
3T3 小鼠胚胎成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L929 小鼠成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
3T3/e 小鼠成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Mo-MuLV/3T3 Mo-MuLV感染的3T3 品牌ATCC 规格25毫升/株. 货期1-2周
3T3TK 小鼠成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
NIH3T3 NIH SWISS 小鼠胚细胞 品牌ATCC 规格25毫升/株. 货期1-2周
3T6 小鼠胚胎成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PA317 小鼠胚胎成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Ana-1 小鼠巨噬细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SRSV/3T3 SRSV转化的3T3 品牌ATCC 规格25毫升/株. 货期1-2周
CTLL-2 小鼠T细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CHL 中国仓鼠肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BRL 肝细胞 品牌ATCC 规格25毫升/株. 货期1-2周
NRK 鼠肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L-6TG 肌母细胞 品牌ATCC 规格25毫升/株. 货期1-2周
V79 中国仓鼠肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BHK 幼仓鼠肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CHO 中国仓鼠卵巢细胞 品牌ATCC 规格25毫升/株. 货期1-2周
R1610 中国仓鼠体细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CHO/dhFr- 二氢叶酸还原酶缺陷型CHO细胞 品牌ATCC 规格25毫升/株. 货期1-2周
2BS 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HLF 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
WI-38 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SL-7 胚肺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
XJH B**细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L-02 肝细胞 品牌ATCC 规格25毫升/株. 货期1-2周