产品名称：小鼠脑干星形胶质细胞

收到小鼠脑干星形胶质细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠脑干星形胶质细胞Description

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions for their partner neurons in the central nervous system, such as neuronal guidance during development, ion and water homeostasis, blood flow regulation, neurotransmission, energy metabolism, and immune defense [1]. Astrocytes have also been implicated in various pathological processes [2]. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release [3]. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS [4]. Cultured mouse astrocytes are a useful in vitro model for studying the molecular and cellular properties of the central nervous system.

MA-bs from ScienCell Research Laboratories are isolated from embryonic day 15 mouse brain stem. MA-bs are cryopreserved as primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MA-bs are characterized by immunofluorescence with antibody specific to GFAP. MA-bs are negative for mycoplasma, bacteria, yeast, and fungi. MA-bs are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing MA-bs in vitro.

Product Use

MA-bs are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Oberheim N, Goldman S, Nedergaard M. (2012) "Heterogeneity of astrocytic form and function." Methods in Mol Biol. 814: 23-45.

[2] van der Laan LJ, De Groot CJ, Elices MJ, Dijkstra CD. (1997) "Extracellular matrix proteins expressed by human ***** astrocytes in vivo and in vitro: an astrocyte surface protein containing the CS1 domain contributes to binding of lymphoblasts." J Neurosci Res. 50: 539-48.

[3] Chen Y, Swanson RA. (2003) "Astrocytes and brain injury." J Cereb Blood Flow Metab. 23: 137-49. [4] Shao Y, McCarthy KD. (1994) “Plasticity of astrocytes.” Glia. 11: 147-55.

小鼠脑干星形胶质细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠脑干星形胶质细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠脑干星形胶质细胞其它细胞系展示表：Sf-21 昆虫细胞系 品牌ATCC 规格25毫升/株. 货期1-2周
SF-9 昆虫细胞系 品牌ATCC 规格25毫升/株. 货期1-2周
L8824 草鱼肝脏细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CIK 草鱼肾脏细胞 品牌ATCC 规格25毫升/株. 货期1-2周
WCF 团头鲂尾鳍细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SCF 白鲢尾鳍细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SiHa 人宫颈鳞状细胞癌 品牌ATCC 规格25毫升/株. 货期1-2周
Ski 人宫颈上皮细胞癌 品牌ATCC 规格25毫升/株. 货期1-2周
SW626 人卵巢腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BI U－87 膀胱癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C6/36 蚊子细胞 品牌ATCC 规格25毫升/株. 货期1-2周
ybZY－1 大鼠肾小球系膜细胞 品牌ATCC 规格25毫升/株. 货期1-2周
AN3 CA 人**内膜腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
H4 人脑神经胶质瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HEC－1－B 人**膜腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MADB106 大鼠乳腺腺癌细胞系 品牌ATCC 规格25毫升/株. 货期1-2周
yb-hxbz-001 人口腔表皮样癌细胞 KB 规格25毫升/株.
yb-hxbz-002 人口腔上皮癌长春新碱耐药株 KB/V 规格25毫升/株.
yb-hxbz-003 人口腔癌细胞 HB 规格25毫升/株.
yb-hxbz-004 人喉表皮样癌细胞 HEp-2 规格25毫升/株.
yb-hxbz-005 人咽鳞癌细胞 FaDu 规格25毫升/株.
yb-hxbz-006 人喉癌细胞 TU 686 规格25毫升/株.
yb-hxbz-007 人喉癌细胞 TU212 规格25毫升/株.
yb-hxbz-008 人喉癌细胞 M2e 规格25毫升/株.
yb-hxbz-009 人喉癌细胞 M4e 规格25毫升/株.
yb-hxbz-010 人食管癌细胞 Eca-109 规格25毫升/株.
yb-hxbz-011 人食管癌细胞 EC871214 规格25毫升/株.
yb-hxbz-012 人食管癌细胞 CaEs-17 规格25毫升/株.
yb-hxbz-013 人食管癌细胞 TE-1 规格25毫升/株.
yb-hxbz-014 人食管癌细胞 TE-10 规格25毫升/株.