产品名称：小鼠小脑星形胶质细胞

收到小鼠小脑星形胶质细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠小脑星形胶质细胞Description

Astrocytes are the majority cell type of the mammalian brain. Astrocytes have been implicated in a variety of supportive functions for their partner neurons in the CNS, such as neuronal guidance during development, and nutritional and metabolic support throughout life [1]. The functions of astroyctes are also complicated during pathological processes [2]. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS [3]. Much of what we have learned about astrocytes is from in vitro studies and astrocytes culture is continually providing a useful tool in exploring the diverse property of astrocytes.

MA-c from ScienCell Research Laboratories are isolated from neonate day two mouse brain (cerebellar cortex). MA-c are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MA-c are characterized by immunofluorescent method with antibody to GFAP. MA-c are negative for mycoplasma, bacteria, yeast and fungi. MA-c are guaranteed to further expand for 5 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. No. 1831) for the culturing of MA-c in vitro.

Product Use

MA-c are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Astrocytes, pharmacology and function. Edited by Sean Murphy. 1993 by Academic press, Inc.

[2] Van der Laan, L. J. W., De Groot, C. J. A., Elices, M. J. and Dijkstran, C. D. (1997) Extracellular matrix proteins expressed by human ***** astrocytes in vivo and in vitro: an astrocyte surface protein containing the CS1 domain contributes to binding of lymphoblasts. J. Neurosci. Res. 50:539-548.

[3] Shao, Y. and McCarhy, K. D. (1994) Plasticity of astrocytes. Glia 11:147-155.

小鼠小脑星形胶质细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠小脑星形胶质细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠小脑星形胶质细胞其它细胞系展示表：yb-hxbz-067 人盲肠腺癌细胞（未分化） Hce-8693 规格25毫升/株.
yb-hxbz-068 人结直肠腺癌细胞 HCT-15 规格25毫升/株.
yb-hxbz-069 人结直肠腺癌细胞 LS174T 规格25毫升/株.
yb-hxbz-070 人结肠腺癌细胞 LS180 规格25毫升/株.
yb-hxbz-071 人高分化结肠腺癌细胞系 THC-8307 规格25毫升/株.
yb-hxbz-072 人结直肠腺癌上皮细胞 DLD-1 规格25毫升/株.
yb-hxbz-073 人小肠癌细胞系 HIC 规格25毫升/株.
yb-hxbz-074 人结肠癌细胞 HRT-S1 规格25毫升/株.
yb-hxbz-075 人直肠腺癌细胞系 HRC-99 规格25毫升/株.
yb-hxbz-076 人胰腺癌细胞 PANC-1 规格25毫升/株.
yb-hxbz-077 人胰腺癌细胞 CFPAC-1 规格25毫升/株.
yb-hxbz-078 人胰腺癌细胞 PC-3 规格25毫升/株.
yb-hxbz-079 人转移胰腺癌细胞 AsPc-1 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-hxbz-080 人原位胰腺腺癌细胞 BxPC-3 规格25毫升/株.
yb-hxbz-081 人胰腺细胞 AR42J 规格25毫升/株.
yb-hxbz-082 人胰腺癌细胞 MIApaca-2 规格25毫升/株.
yb-hxbz-083 人胰腺癌细胞 JF-305 规格25毫升/株.
yb-hxbz-084 人胰腺癌细胞 PC-2 规格25毫升/株.
yb-hxbz-085 人胰腺癌细胞 SW1990 规格25毫升/株.
yb-hxbz-086 人胰腺癌细胞 CaPan-Ⅱ/Capan-2 规格25毫升/株.
yb-hxbz-087 人胰腺癌细胞 CRL 规格25毫升/株.
yb-hxbz-088 人胰腺癌细胞 SFPAC-1 规格25毫升/株.
yb-hxbz-089 人胚胎胰腺组织来源细胞 CCC-HPE-2 规格25毫升/株.
yb-hxbz-090 人胰腺腺泡上皮癌 HPAC 规格25毫升/株.
yb-hxbz-091 人胰腺腺癌细胞 Panc 10.05 规格25毫升/株.
yb-hxbz-092 人肝癌细胞 HepG-2 规格25毫升/株.
yb-hxbz-093 人肝癌细胞 HepG2.2.1.5 规格25毫升/株.
yb-hxbz-094 人肝癌细胞 SMMC-7721 规格25毫升/株.