产品名称：小鼠神经束膜细胞

收到小鼠神经束膜细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠神经束膜细胞Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic condition, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles [1]. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100 [3].

MPNF from ScienCell Research Laboratories are isolated from postnatal day 8 mouse sciatic nerve. MPNF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MPNF are characterized by immunofluorescence with antibodies specific to vimentin, S-100, GFAP and CD90. MPNF are negative for mycoplasma, bacteria, yeast, and fungi. MPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPNF in vitro.