产品名称：小鼠星形胶质细胞

收到小鼠星形胶质细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠星形胶质细胞Description

Astrocytes are the majority cell type of the mammalian brain. Astrocytes have been implicated in a variety of supportive functions for their partner neurons in the CNS, such as neuronal guidance during development and nutritional and metabolic support throughout life [1]. The functions of astroyctes are also complicated during pathological processes [2]. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS [3]. Much of what we have learned about astrocytes is from in vitro studies and astrocyte culture is continually providing a useful tool in exploring the diverse property of astrocytes.

MA from ScienCell Research Laboratories are isolated from neonate day two mouse brain (cerebral cortex). MA are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MA are characterized by immunofluorescent method with antibody to GFAP. MA are negative for mycoplasma, bacteria, yeast and fungi. MA are guaranteed to further expand for 5 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. No. 1831) for the culturing of MA in vitro.

Product Use

MA are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Astrocytes, pharmacology and function. Edited by Sean Murphy. 1993 by Academic press, Inc.

[2] Van der Laan, L. J. W., De Groot, C. J. A., Elices, M. J. and Dijkstran, C. D. (1997) Extracellular matrix proteins expressed by human ***** astrocytes in vivo and in vitro: an astrocyte surface protein containing the CS1 domain contributes to binding of lymphoblasts. J. Neurosci. Res. 50:539-548.

[3] Shao, Y. and McCarhy, K. D. (1994) Plasticity of astrocytes. Glia 11:147-155.