产品名称：小鼠雪旺细胞

收到小鼠雪旺细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠雪旺细胞Description

Schwann cells are neural crest derivatives that ensheathe and myelinate axons of peripheral nerves [1]. Each Schwann cell wraps around the shaft of an individual peripheral axon, forming myelin sheaths along segments of the axon. Schwann cells play important roles in the development, function, and regeneration of peripheral nerves. When an axon is dying, the Schwann cells surrounding it aid in its digestion, leaving an empty channel formed by successive Schwann cells, through which a new axon may then grow from a severed end. The number of Schwann cells in peripheral nerves is tightly regulated [2]. Their proliferation in vitro can be stimulated by various growth factors including PDGF, FGF, neuregulin, and others [3]. Schwann cells provide a relatively simple, well-defined, and accessible mammalian model for the study of a number of developmental questions. It is also of particular clinical importance in understanding the biology of Schwann cells, not only in the context of neuropathies and nerve regeneration, but also because the cells or their precursors may be especially well suited for implants to facilitate repair in the CNS.

MSC from ScienCell Research Laboratories are isolated from neonatal mouse sciatic nerves. MSC are cryopreserved either at primary or passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MSC are characterized by immunofluorescence with antibodies specific to S-100, GFAP, and CD90. MSC are negative for mycoplasma, bacteria, yeast, and fungi. MSC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Schwann Cell Medium (SCM, Cat. No. 1701) for the culturing of MSC in vitro.

Product Use

MSC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Jessen, K. R. and Mirsky, R. (1999) Schwann cells and their precursors emerge as major regulators of nerve development. Trends Neurosci. 22:402-410.

[2] Syroid, D. E., Maycox, P. R., Burrola, P. G., Liu, N., Wen, D., Lee, K. F., Lemke, G., Kilpatrick, T. J. (1996) Cell death in the Schwann cell lineage and its regulation by neuregulin. Proc. Natl. Acad. Sci. USA 93:9229-9234.

[3] Rahmatullah, M., Schroering, A., Rothblum, K., Stahl, R. C., Urban, B and Carey, D. J. (1998) Synergistic regulation of Schwann cells proliferation by heregulin and forskolin. Mol. Cell. Biol. 18:6245-6252.

小鼠雪旺细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠雪旺细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠雪旺细胞其它细胞系展示表：yb-hxbz-144 人**细胞与仓鼠肺细胞杂交细胞 FD4 规格25毫升/株.
yb-hxbz-145 人**细胞与仓鼠肺细胞杂交细胞 FD6 规格25毫升/株.
yb-hxbz-146 人小细胞肺癌细胞 NCI-H446 规格25毫升/株.
yb-hxbz-147 人小细胞肺癌细胞 NCI-H345 规格25毫升/株.
yb-hxbz-148 人小细胞肺癌细胞 DMS153 规格25毫升/株.
yb-hxbz-149 人经典小细胞肺癌细胞 NCI-H1688 规格25毫升/株.
yb-hxbz-150 人大细胞肺癌细胞 NCI-H460 规格25毫升/株.
yb-hxbz-151 人大细胞肺癌细胞 NCI-H661 规格25毫升/株.
yb-hxbz-152 人肺鳞癌细胞 NCI-H520 规格25毫升/株.
yb-hxbz-153 人肺支气管癌细胞 NCI-H1650 规格25毫升/株.
yb-hxbz-154 人非小细胞肺腺癌细胞 NCI-H157 规格25毫升/株.
yb-hxbz-155 人非小细胞肺腺癌细胞 NCI-H1975 规格25毫升/株.
编号 中文 英文名称 报价
yb-hxbz-156 人非小细胞肺癌细胞 NCI-H838 规格25毫升/株.
yb-hxbz-157 人肺小细胞肺癌 NCI-H2227 规格25毫升/株.
yb-hxbz-158 人肺癌细胞（**结转移） NCI-H292 规格25毫升/株.
yb-hxbz-159 人肺腺鳞癌细胞 NCI-H596 规格25毫升/株.
yb-hxbz-160 人非小细胞肺癌细胞 NCI-H23 规格25毫升/株.
yb-hxbz-161 人非小细胞肺腺癌细胞 NCI-H2087 规格25毫升/株.
yb-hxbz-162 人肺腺癌细胞 A549 规格25毫升/株.
yb-hxbz-163 人肺癌细胞 A-427 规格25毫升/株.
yb-hxbz-164 人肺癌细胞 H125 规格25毫升/株.
yb-hxbz-165 人肺巨细胞癌 PG 规格25毫升/株.