产品名称：CD1小鼠垂体细胞

收到CD1小鼠垂体细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

CD1小鼠垂体细胞Brain Vascular Pericytes (BVP) are perivascular cells that are closely associated with the endothelium of capillaries and other small vessels [1]. BVP, located between endothelial cells and astrocytes in the brain, communicate with other cells by extending long cytoplasmic processes which wrap around the capillaries [1, 2]. BVP participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the blood-brain barrier [3, 4]. Pericyte dysregulation has been linked to several pathological conditions such as hypertension, diabetic retinopathy, atherosclerosis, multiple sclerosis, Alzheimer’s disease, and tumor angiogenesis [2, 4]. The unique and diverse functions of BVP make them novel candidates for cell therapy in regenerative medicine. Cultured primary mouse BVP (MBVP) are a useful in vitro model for understanding the molecular mechanisms of blood-brain barrier regulation and for studying a wide variety of central nervous system diseases.

MBVP from ScienCell Research Laboratories are isolated from ***** mouse brain. MBVP are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MBVP are characterized by immunofluorescence with antibody specific to Neural/glial antigen 2 (NG2) and -smooth muscle actin. MBVP are negative for mycoplasma, bacteria, yeast, and fungi. MBVP are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Pericyte Medium (PM, Cat. #1201) for culturing MBVP in vitro

CD1小鼠垂体细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，CD1小鼠垂体细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
CD1小鼠垂体细胞其它细胞系展示表：yb-hxbz-252 人乳腺癌细胞 MCF-7(NEW) 规格25毫升/株.
yb-hxbz-253 人乳腺癌细胞 MCF-7(OLD) 规格25毫升/株.
yb-hxbz-254 人乳腺癌细胞 MCF-7/ER36 规格25毫升/株.
yb-hxbz-255 人乳腺癌细胞(耐阿霉素) MCF-7/ADR 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-hxbz-256 人乳腺癌细胞 MCF-7B 规格25毫升/株.
yb-hxbz-257 人乳腺癌细胞 SKBr-2HL 规格25毫升/株.
yb-hxbz-258 人乳腺癌细胞 ZR75-1 规格25毫升/株.
yb-hxbz-259 人乳腺癌细胞 ZR75-30 规格25毫升/株.
yb-hxbz-260 人乳腺癌细胞 MDA-MB-231 规格25毫升/株.
yb-hxbz-261 人乳腺癌细胞 MDA-MB-468 规格25毫升/株.
yb-hxbz-262 人乳腺癌细胞 Hs-578T 规格25毫升/株.
yb-hxbz-263 人乳腺癌细胞 HCC1937 规格25毫升/株.
yb-hxbz-264 人乳腺癌细胞 MDA-MB-415 规格25毫升/株.
yb-hxbz-265 人乳腺癌高转移细胞 MDA-MB-435 规格25毫升/株.
yb-hxbz-266 人乳腺癌细胞 MDA-MB-435S 规格25毫升/株.
yb-hxbz-267 人乳腺癌细胞 MDA-MB-436 规格25毫升/株.
yb-hxbz-268 人乳腺导管癌细胞 MDA-MB-453 规格25毫升/株.
yb-hxbz-269 人乳腺细胞 HBL-100 规格25毫升/株.
yb-hxbz-270 人乳腺癌细胞 BCaP-37 规格25毫升/株.
yb-hxbz-271 人乳腺癌细胞 LCC1 规格25毫升/株.
yb-hxbz-272 人乳腺癌细胞 MX-1 规格25毫升/株.
yb-hxbz-273 人乳腺管癌细胞 T47D 规格25毫升/株.
yb-hxbz-274 人乳腺癌细胞 SK-BR-3 规格25毫升/株.
yb-hxbz-275 人乳腺癌细胞 4T1 规格25毫升/株.
yb-hxbz-276 人乳腺癌细胞 HCC1937 规格25毫升/株.