产品名称：猪角膜上皮细胞

收到猪角膜上皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

猪角膜上皮细胞Description

The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue. The cornea consists of three distinct cell layers: the outer epithelium, the inner endothelium and the central stroma (the keratocytes). The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals that activate the corneal defense system [1]. It also highly expresses aldehyde dehydrogenase to protect against UV- and 4-hydroxynonenal-induced cellular damage [2]. Corneal epithelial basal cell proliferation is controlled by a host of cytokines, e.g., epidermal growth factor, that activate their cognate receptors in the deeper layers of the epithelium [3]. Instead of testing products in the eyes of animals, cultured corneal epithelial cells could be used for toxicology tests in in vitro models and for studying the molecular mechanisms involved in regulating human corneal epithelial cell differentiation.

PCEpiC from ScienCell Research Laboratories are isolated from porcine cornea. PCEpiC are cryopreserved from passage one culture. Each vial contains >5 x 10^5 cells in 1 ml volume. PCEpiC are characterized by their cobble stone morphology serum-free culture and immunofluorescent method with antibody to CK-18 and -19. PCEpiC are negative mycoplasma, bacteria, yeast and fungi. PCEpiC are guaranteed to further expand for 10 population doublings at the condition provided by ScienCell Research Laboratories.

Product Use

PCEpiC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Zhang, J., Wu, X. Y. and Yu, F. S. (2005) Inflammatory response of corneal epithelial cells to pseudomonas aeruginosa infection. Curr. Eye Res. 30:527-534.

[2] Pappa, A., Brown, D., Koutalos, Y., Degregori, J., White, C. and Vasiliou, V. (2005) Human aldehyde dehydrogenase 3A1 inhibits proliferation and promotes survival of human corneal epithelial cells. J. Biol. Chem. 280:27998-28006.

[3]. Yang, H., Mergler, S., Sun, X., Wang, Z, Lu, L., Bonanno, J. A., Pleyer, U. and Reinach, P. S. (2005). TRPC4 knockdown suppresses EGF-induced store operated channel activation and growth in human corneal epithelial cells. J Biol Chem. 280: 32230-7.

猪角膜上皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，猪角膜上皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
猪角膜上皮细胞其它细胞系展示表：yb1009 HN Human Neurons 10 x 10^6 cells/vial ATCC
yb1010 HN Human Neurons 5 x 10^6 cells/vial ATCC
yb1011 HCGC 人小脑颗粒细胞 Human Cerebellar Granule Cells 1 x 10^6 cells/vial ATCC
yb1012 HN-h 人海马趾神经元 Human Neurons-hippocampal 1 x 10^6 cells/vial ATCC
yb1013 HCN 人大脑皮层神经元 Human Cortical Neurons 1 x 10^6 cells/vial ATCC
yb1014 HN-bs 人脑干神经元 Human Neurons-brain stem 1 x 10^6 cells ATCC
yb1015 HOPC 人少突胶质前体细胞--贴壁生长 Human Oligodendrocyte Precursor Cells 1 x 10^6 cells/vial ATCC
yb1016 HOPC-os 人少突胶质前体细胞--悬浮生长 Human Oligodendrocyte Precursor Cells-oligospheres 1 x 10^6 cells/vial ATCC
yb1017 HO 人少突胶质细胞 Human Oligodendrocytes 1 x 10^6 cells/vial ATCC
yb1018 HOPC-f 人少突胶质前体细胞--新鲜 Human Oligodendrocyte Precursor Cells-fresh 10 x 10^6 cells/tube ATCC
yb1019 HOPC-f40 Human Oligodendrocyte Precursor Cells-fresh 40 x 10^6 cells/tube ATCC
yb1020 HOPC-f80 Human Oligodendrocyte Precursor Cells-fresh 80 x 10^6 cells/tube ATCC
yb1021 HOPC-f20 Human Oligodendrocyte Precursor Cells-fresh 20 x 10^6 cells/tube ATCC
yb1022 HSC 人雪旺细胞 Human Schwann Cells 5 x 10^5 cells/vial ATCC
yb1023 HPC 人神经束膜细胞 Human Perineurial Cells 5 x 10^5 cells/vial ATCC
yb1024 HA 人星形胶质细胞 Human Astrocytes 1 x 10^6 cells/vial ATCC
yb1025 HA-c 人小脑星形胶质细胞 Human Astrocytes-cerebellar 1 x 10^6 cells/vial ATCC
yb1026 HA-sp 人脊髓星形胶质细胞 Human Astrocytes-spinal cord 1 x 10^6 cells/vial ATCC
yb1027 HA-h 人海马星形胶质细胞 Human Astrocytes-hippocampal 1 x 10^6 cells/vial ATCC
yb1028 HA-bs 人星形胶质细胞--脑干 Human Astrocytes-brain stem 5 x 10^5 cells/vial ATCC
yb1029 HA-mb 人星形胶质细胞 - 中脑 Human Astrocytes - middle brain 5 x 10^5 cells/vial ATCC
yb1030 HRA 人类视网膜星形胶质细胞 Human Retinal Astrocytes 5 x 10^5 cells/vial ATCC
yb1031 HM 人小神经胶质细胞 Human Microglia 5 x 10^5 cells/vial ATCC
yb1032 ATCC