产品名称：牛脑微血管内皮细胞

收到牛脑微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

牛脑微血管内皮细胞Description

Brain microvascular endothelial cells (BMEC), the major component of the blood-brain barrier, limit the passage of substances, both soluble and cellular, from the blood into the brain. BMEC utilize unique features to distinguish themselves from peripheral endothelial cells, such as 1) intercellular tight junctions that display high electrical resistance and slow paracellular transport, 2) the absence of fenestrae and a reduced level of pinocytic activity, and 3) the expression of specialized pumps that can transport compounds out of the brain via the blood-brain barrier [1-3]. Similar to peripheral endothelial cells, BMEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. Cultured bovine BMEC have been used for studying the molecular and cellular properties of blood-brain barrier because of their unique functions [4]. Understanding the molecular mechanisms of blood-brain barrier regulation may help to optimize drug delivery to the CNS and elucidate new therapies for CNS diseases.

BBMEC from ScienCell Research Laboratories are isolated from bovine brain. BBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. BBMEC are characterized by immunofluorescence with antibody specific to vWF/Factor VIII. BBMEC are negative for mycoplasma, bacteria, yeast, and fungi. BBMEC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing BBMEC in vitro.

Product Use

BBMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Crone C, Oleson SP. (1992) "Electrical resistance of brain microvessel endothelium." Brain Res. 241: 49-55.

[2] Reese TS, Karnovsky MJ. (1967) "Fine structural localization of blood-brain barrier to exogenous peroxidase." J Cell Biol. 34: 9-14.

[3] Wolburg H, Neuhaus J, Kniesel U, Kraub B, Schmid EM, Ocalan M, Farrell C, Risau W. (1994) "Modulation of tight junction structure in blood-brain barrier endothelial cells." J Cell Sci. 107: 1347-1357.

[4] Dorovini-Zis K, Bowman P, Betz A, Goldstein G. (1987) "Formation of a barrier by brain microvessel endothelial cells in culture." Fed Proc. 46(8): 2521-2522.

牛脑微血管内皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，牛脑微血管内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
牛脑微血管内皮细胞其它细胞系展示表：yb-hxbz-243 人肾癌细胞 SK-RC-42 规格25毫升/株.
yb-hxbz-244 人膀胱癌细胞 EJ 规格25毫升/株.
yb-hxbz-245 人膀胱癌细胞 5637（HTB-9） 规格25毫升/株.
yb-hxbz-246 人膀胱移行细胞癌细胞 T24   规格25毫升/株.
yb-hxbz-247 人膀胱鳞癌细胞 ScaBER 规格25毫升/株.
yb-hxbz-248 人膀胱癌细胞 BIU－87 规格25毫升/株.
人生殖系统细胞株
yb-hxbz-249 人胆管癌细胞 RBE 规格25毫升/株.
yb-hxbz-250 人胆管癌细胞 QBC939 规格25毫升/株.
yb-hxbz-251 人乳腺癌细胞 MCF-7 规格25毫升/株.
yb-hxbz-252 人乳腺癌细胞 MCF-7(NEW) 规格25毫升/株.
yb-hxbz-253 人乳腺癌细胞 MCF-7(OLD) 规格25毫升/株.
yb-hxbz-254 人乳腺癌细胞 MCF-7/ER36 规格25毫升/株.
yb-hxbz-255 人乳腺癌细胞(耐阿霉素) MCF-7/ADR 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-hxbz-256 人乳腺癌细胞 MCF-7B 规格25毫升/株.
yb-hxbz-257 人乳腺癌细胞 SKBr-2HL 规格25毫升/株.
yb-hxbz-258 人乳腺癌细胞 ZR75-1 规格25毫升/株.
yb-hxbz-259 人乳腺癌细胞 ZR75-30 规格25毫升/株.
yb-hxbz-260 人乳腺癌细胞 MDA-MB-231 规格25毫升/株.
yb-hxbz-261 人乳腺癌细胞 MDA-MB-468 规格25毫升/株.
yb-hxbz-262 人乳腺癌细胞 Hs-578T 规格25毫升/株.
yb-hxbz-263 人乳腺癌细胞 HCC1937 规格25毫升/株.
yb-hxbz-264 人乳腺癌细胞 MDA-MB-415 规格25毫升/株.
yb-hxbz-265 人乳腺癌高转移细胞 MDA-MB-435 规格25毫升/株.
yb-hxbz-266 人乳腺癌细胞 MDA-MB-435S 规格25毫升/株.
yb-hxbz-267 人乳腺癌细胞 MDA-MB-436 规格25毫升/株.
yb-hxbz-268 人乳腺导管癌细胞 MDA-MB-453 规格25毫升/株.
yb-hxbz-269 人乳腺细胞 HBL-100 规格25毫升/株.
yb-hxbz-270 人乳腺癌细胞 BCaP-37 规格25毫升/株.
yb-hxbz-271 人乳腺癌细胞 LCC1 规格25毫升/株.
yb-hxbz-272 人乳腺癌细胞 MX-1 规格25毫升/株.
yb-hxbz-273 人乳腺管癌细胞 T47D 规格25毫升/株.
yb-hxbz-274 人乳腺癌细胞 SK-BR-3 规格25毫升/株.
yb-hxbz-275 人乳腺癌细胞 4T1 规格25毫升/株.
yb-hxbz-276 人乳腺癌细胞 HCC1937 规格25毫升/株.
yb-hxbz-277 人乳腺导管癌细胞 HCC38 规格25毫升/株.
yb-hxbz-278 人乳腺癌细胞 Hs-578T 规格25毫升/株.
yb-hxbz-279 人乳腺癌Tamoxifen耐药株 LCC2 规格25毫升/株.
yb-hxbz-280 人乳腺癌抗雌**耐药株 LCC9 规格25毫升/株.
yb-hxbz-281 人乳腺癌阿霉素耐药细胞株 MCF/Adr 规格25毫升/株.
yb-hxbz-282 人乳腺癌细胞 BT-549 规格25毫升/株.
yb-hxbz-283 人乳腺导管瘤细胞 BT-474 规格25毫升/株.
yb-hxbz-284 人乳腺癌细胞 MB-271 规格25毫升/株.