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吲哚胺2,3-双加氧酶(IDO)重组蛋白
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 片段与标签Ala2~Gly403 with N-terminal His Tag宿主E.coli
- 内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量46.7kDa
- 实际分子量43kDa(差异分析请参阅说明书)
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 97%
- 等电点6.8
-
应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
吲哚胺2,3-双加氧酶(IDO)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
吲哚胺2,3-双加氧酶(IDO)重组蛋白
Recombinant Indoleamine-2,3-Dioxygenase (IDO)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
吲哚胺2,3-双加氧酶(IDO)重组蛋白[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ala2~Gly403
Tags: N-terminal His-Tag
Tissue Specificity: Lung, Blood. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 46.7kDa
Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
吲哚胺2,3-双加氧酶(IDO)重组蛋白[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
Figure 2. SDS-PAGE
