产品名称：早幼粒细胞白血病蛋白(PML)重组蛋白

早幼粒细胞白血病蛋白(PML)重组蛋白

物种Homo sapiens (Human，人)相同的名称，不同的物种。

• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量26.2kDa
• 实际分子量-(差异分析请参阅说明书)
• 片段与标签Gln59~Glu239 (Accession # P29590) with N-terminal His Tag
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  早幼粒细胞白血病蛋白(PML)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  早幼粒细胞白血病蛋白(PML)重组蛋白

  
  

  Promyelocytic Promyelocytic Promyelocytic Promyelocytic Leukemia Leukemia Leukemia Leukemia Protein Protein Protein Protein (PML)

  Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

  Instruction Instruction Instruction Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

  4th Edition (Revised in August, 2012)

  早幼粒细胞白血病蛋白(PML)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

  Protein Protein Protein Protein Names: Promyelocytic Leukemia Protein

  Synonyms: Synonyms: Synonyms: Synonyms: PML, MYL, RNF71, TRIM19

  Species: Species: Species: Species: Human

  Size: 1mg

  Source: Source: Source: Source: Escherichia coli-derived

  Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus; nucleoplasm; Cytoplasm; Endoplasmic reticulum

  membrane; Peripheral membrane protein; Cytoplasmic side; Early endosome

  membrane.

  [ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

  Residues Residues Residues Residues: Gln59~Glu239 (Accession # P29590), with two N-terminal Tags, His-tag and

  S-tag.

  Grade & Purity: Purity: Purity: Purity: >92%, 26 kDa as determined by SDS-PAGE reducing conditions.

  Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl, 5% sucrose.

  Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 26.2 kDa

  Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.9

  早幼粒细胞白血病蛋白(PML)重组蛋白[ PREPARATION PREPARATION PREPARATION PREPARATION ]

  Reconstitute in sterile PBS, pH7.2-pH7.4.

  [ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

  Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

  Store at 2-8oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

  determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious

  degradation and precipitation were observed. (Referring from China Biological Products Standard, which was

  calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

  appropriate storage condition.

  [ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

  The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.

  MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-QC QAEAKCPKLL PCLHTLCSGC

  LEASGMQCPI CQAPWPLGAD TPALDNVFFE SLQRRLSVYR QIVDAQAVCT RCKESADFWC FECEQLLCAK CFEAHQWFLK

  HEARPLAELR NQSVREFLDG TRKTNNIFCS NPNHRTPTLT SIYCRGCSKP LCCSCALLDS SHSELKCDIS AEIQQRQEE

  [ REFERENCES REFERENCES REFERENCES REFERENCES ]

  1. Shen T.H., et al. (2006) Mol. Cell 24:331-339.

  2. Gresko E., et al. (2009) Oncogene 28:698-708.

  3. Cuchet D., et al. (2011) J. Cell Sci. 124:280-291.

  4. Jensen K., et al. (2001) Oncogene 20:7223-7233.