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真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 性状冻干粉内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量13.9kDa
- 实际分子量-(差异分析请参阅说明书)
- 片段与标签Ser2-Ile118 (Accession # Q13541) with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 纯度> 95%
- 等电点-
-
应用SDS-PAGE; WB; ELISA; I
真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白
Eukaryotic Eukaryotic Eukaryotic Eukaryotic Translation Translation Translation Translation Initiation Initiation Initiation Initiation Factor 4E Binding Binding Binding Binding Protein Protein Protein Protein 1 (EIF4EBP1) (EIF4EBP1) (EIF4EBP1) (EIF4EBP1)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
1th Edition (Revised in February, 2012)
真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Eukaryotic Translation Initiation Factor 4E Binding Protein 1
Gene Names: EIF4EBP1
Size: 100µg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Regulates eIF4E activity by preventing its assembly into the eIF4F complex.
Mediates the regulation of protein translation by hormones, growth factors and other
stimuli that signal through the MAP kinase and mTORC1 pathways.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus but not nucleoli, Cytoplasm
Tissue Specificity: Specificity: Specificity: Specificity: Selective cytoplasmic expression in salivary gland, pancreas, the
gastrointestinal tract and non-keratinized squamous epithelia.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Ser2-Ile118 (Accession # Q13541), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 13.97 kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 13.97 kDa
真核翻译起始因子4E结合蛋白1(EIF4EBP1)重组蛋白[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGSEF-SGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLM
ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Schalm S.S., et al. (2003) Curr. Biol. 13:797-806. 2. Pause A., et al. (1994) Nature 371:762-767. 3. Ha, Sang Hoon, et al. (2006) Cell. Signal. (England) 18 (12): 2283–2291.
4. Haghighat A., et al. (1995) EMBO J. 14:5701-5709. 5. Hara K., et al. (2002) Cell 110:177-189.
