产品名称：整合素α8(ITGa8)重组蛋白

整合素α8(ITGa8)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 片段与标签Asp907~Ala1063 with N-terminal His and GST Tag宿主E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位细胞膜
• 预测分子量47.6kDa
• 实际分子量52kDa(差异分析请参阅说明书)
• 缓冲液成份100mM NaHCO3, 500mM NaCl缓冲液（pH8.3,含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
• 性状冻干粉
• 纯度> 95%
• 等电点7.9
• 应用SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labelin
  整合素α8(ITGa8)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  整合素α8(ITGa8)重组蛋白

  
  

  Recombinant Integrin Alpha 8 (ITGa8)

  Organism Species: Homo sapiens (Human)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  11th Edition (Revised in May, 2016)

  整合素α8(ITGa8)重组蛋白[ PROPERTIES ]

  Source: Prokaryotic expression. Host: E. coli

  Residues: Asp907~Ala1063

  Tags: Two N-terminal Tags, His-tag and GST-tag

  Tissue Specificity: Kidney, Liver. Subcellular Location: Membrane; Single-pass type I membrane protein. Purity: >95%

  Traits: Freeze-dried powder

  Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

  EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

  Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

  Labeling. (May be suitable for use in other assays to be determined by the end user.)

  Predicted isoelectric point: 7.9

  Predicted Molecular Mass: 47.6kDa

  Accurate Molecular Mass: 52kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

  The possible reasons that the actual band size differs from the predicted are as

  follows:

  1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

  2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

  give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]

  Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of

  0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

  oC for one month. Aliquot and store at -80

  oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

  was determined by accelerated thermal degradation test, that is, incubate the

  protein at 37

  oC for 48h, and no obvious degradation and precipitation were

  observed.The loss rate is less than 5% within the expiration date under

  appropriate storage condition. [ SEQUENCE ]

  整合素α8(ITGa8)重组蛋白[ IDENTIFICATION ]

  Figure 1. SDS-PAGE