产品名称：整合素β1(ITGb1)重组蛋白

整合素β1(ITGb1)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 片段与标签Asp140~Ile383 (Accession # P05556) with N-terminal His Tag宿主E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量59.1kDa
• 实际分子量-(差异分析请参阅说明书)
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  整合素β1(ITGb1)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  整合素β1(ITGb1)重组蛋白

  
  

  Recombinant Integrin Beta 1 (ITGb1)

  Organism Species: Homo sapiens (Human)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  9th Edition (Revised in Jul, 2013)

  整合素β1(ITGb1)重组蛋白[ PROPERTIES ]

  Residues: Asp140~Ile383 (Accession # P05556),

  with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

  Subcellular Location: Cell membrane;

  Single-pass type I membrane protein.

  Melanosome.

  Purity: >95%

  Endotoxin Level: <1.0EU per 1μg

  (determined by the LAL method).

  Formulation: Supplied as lyophilized form in PBS,

  pH7.4, containing 1mM DTT, 5% trehalose, 0.05%

  sarcosyl and preservative.

  Predicted isoelectric point: 5.7

  Predicted Molecular Mass: 59.1kDa

  Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  [ USAGE ]

  Reconstitute in sterile PBS, pH7.2-pH7.4.

  整合素β1(ITGb1)重组蛋白[ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles.

  Store at 2-8oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Test: The thermal stability is described by the loss rate of the target

  protein. The loss rate was determined by accelerated thermal degradation test,

  that is, incubate the protein at 37oC for 48h, and no obvious degradation and

  precipitation were observed. (Referring from China Biological Products Standard,

  which was calculated by the Arrhenius equation.) The loss of this protein is less

  than 5% within the expiration date under appropriate storage condition.

  整合素β1(ITGb1)重组蛋白[ SEQUENCES ]

  The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its

  sequence is listed below.

  MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

  GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

  DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

  KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

  GSTAIGMKET AAA KFE RQ HM DSP D LG T LEV LFQ GP LGSEF - D YPI D LYY LM D

  LSYSMKDDLE NVKSLGTDLM NEMRRITSDF RIGFGSFVEK TVMPYISTTP AKLRNPCTSE

  QNCTSPFSYK NVLSLTNKGE VFNELVGKQR ISGNLDSPEG GFDAIMQVAV CGSLIGWRNV

  TRLLVFSTDA GFHFAGDGKL GGIVLPNDGQ CHLENNMYTM SHYYDYPSIA HLVQKLSENN

  IQTIFAVTEE FQPVYKELKN LIPKSAVGTL SANSSNVIQL IIDAYNSLSS EVI

  [ REFERENCES ]

  1. Argraves W.S., et al. (1987) J. Cell Biol. 105:1183-1190. 2. Balzac F., et al. (1994) J. Cell Biol. 127:557-565. 3. Altruda F., et al. (1990) Gene 95:261-266. 4. Languino L.R., Ruoslahti E. (1992) J. Biol. Chem. 267:7116-7120.