产品名称：中性鞘磷脂酶(NSMASE)重组蛋白

中性鞘磷脂酶(NSMASE)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 片段与标签Lys2~Arg236 (Accession # O60906) with N-terminal His Tag宿主E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量59.2kDa
• 实际分子量-(差异分析请参阅说明书)
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  中性鞘磷脂酶(NSMASE)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  中性鞘磷脂酶(NSMASE)重组蛋白

  
  

  Recombinant Neutral Sphingomyelinase (NSMASE)

  Organism Species: Homo sapiens (Human)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  10th Edition (Revised in Jan, 2014)

  中性鞘磷脂酶(NSMASE)重组蛋白[ PROPERTIES ]

  Residues: Lys2~Arg236

  Tags: Two N-terminal Tags, His-tag and GST-tag

  Accession: O60906

  Host: E. coli

  Subcellular Location: Membrane; Multi-pass

  membrane protein.

  Purity: >95%

  Endotoxin Level: <1.0EU per 1μg

  (determined by the LAL method).

  Formulation: Supplied as lyophilized form in 20mM Tris,

  150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

  0.01% sarcosyl, 5% trehalose, and preservative.

  Predicted isoelectric point: 6.6

  Predicted Molecular Mass: 59.2kDa

  Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  [ USAGE ]

  Reconstitute in ddH2O.

  中性鞘磷脂酶(NSMASE)重组蛋白[ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles.

  Store at 2-8oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Test: The thermal stability is described by the loss rate of the target

  protein. The loss rate was determined by accelerated thermal degradation test,

  that is, incubate the protein at 37oC for 48h, and no obvious degradation and

  precipitation were observed. (Referring from China Biological Products Standard,

  which was calculated by the Arrhenius equation.) The loss of this protein is less

  than 5% within the expiration date under appropriate storage condition.

  [ SEQUENCES ]

  The sequence of the target protein is listed below.

  K P N F S L R L R I F N L N C W G I P Y L S K H R A D R M R R L G D F L N Q E S F D L A L L E E V

  WSEQDFQYLRQKLSPTYPAA HHFRSGIIGS GLCVFSKHPI QELTQHIYTL NGYPYMIHHG

  DWFSGKAVGL LVLHLSGMVL NAYVTHLHAE YNRQKDIYLA HRVAQAWELA QFIHHTSKKA

  DV V L L C G D L N MHP E D L G C C L LK EW T G L H DA Y LE T R D F K GS EE G N TM V P K N

  CYVSQQELKP FPFGVR

  [ REFERENCES ]

  1. Tomiuk S., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:3638-3643. 2. Sawai H., et al. (1999) J. Biol. Chem. 274:38131-38139. 3. Gudbjartsson D.F., et al. (2008) Nat. Genet. 40:609-615. 4. Marchesini N., et al. (2004) J. Biol. Chem. 279:25101-25111.