产品名称：Rabbit anti-mouse Kappa light chain antibody

Rabbit anti-mouse Kappa light chain antibody

英文名称Rabbit anti-mouse Kappa light chain

中文名称兔抗小鼠k链

别    名Ig kappa chain C region; HCAK 1; HCAK1; Ig kappa chain C region; IGKC; Immunoglobulin kappa constant; Immunoglobulin kappa constant region; Immunoglobulin kappa light chain; Kappa 1 immunoglobulin light chain; kappa light chain; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809; IGKC_MOUSE.

Rabbit anti-mouse Kappa light chain antibody

说 明 书1mg  10mg  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Mouse, Rat,

Rabbit anti-mouse Kappa light chain antibody产品应用WB=1:100-500 ELISA=1:200-1000 IHC-P=1:100-500 IHC-F=1:100-500  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量11kDa

性    状Lyophilized or Liquid

浓    度10mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from mouse Ig kappa

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M PBS(pH7.4)

Rabbit anti-mouse Kappa light chain antibody保存条件Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile distilled water or diluent supplied, theantibody is stable for at least two weeks at 2-4 °C.

生 产 商BIOSS

产品介绍background:

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

Gene ID:

16071

Database links:

Entrez Gene: 16071 MOUSE

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Rabbit anti-mouse Kappa light chain antibody产品图片

Tissue/cell: mouse small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;

Incubation: Anti-mouse Kappa light chain Polyclonal Antibody, Unconjugated(bs-0330R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Antigen: bs-0330P, 0.2ug/100ul

Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;

Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;

TMB(C-0024) staining;

Read the data in MicroplateReader by 450nm.