产品名称：人绒毛膜滋养层细胞

收到人绒毛膜滋养层细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人绒毛膜滋养层细胞胚胎滋养层是早期胚泡的外层遮盖物，并做为母亲**内膜和发育中胚胎之间的营养通路。紧贴在**壁的滋养层是胚胎着床必不可少的步骤，随后形成胎盘。覆盖着胎盘绒毛的人绒毛膜滋养层细胞提供母亲循环中氧和营养物质交换的介面。它们合成和释放绒毛膜促性腺**，胎盘催乳**和血管生成素，表达CXCR4, CCR5和泌乳刺**基因家族。在绒毛锚定位点，它们分化成侵袭型并表达CCR1。绒毛膜滋养层细胞的特性及近期发现的滋养层干细胞，使它们成为一个研究细胞分化和器官形成的理想的遗传学平台。    

     人绒毛膜滋养层细胞(HVT)提取于人胚胎绒毛膜滋养层组织，原代冻存。每管含有细胞数>1×106cells/ml，此细胞通过对alpha-和beta- HCG**荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、**、酵母和**。

推荐培养基(TM, Cat. No. 7121)

Reference

[1] Pavlov, N., Hatzi, E., Bassagliam Y., Frendo, J. L., Brion, D. E., Badet, J. (2003) Angiogenin distribution in human term placenta, and expression by cultured trophoblastic cells. Angiogenesis. 6(4):317-30.

[2] Maldonado-Estrada, J., Menu, E., Roques, P., Vaslin, B., Dautry-Varsat, A., Barre-Sinoussi, F., Chaouat, G. (2003) Predominant intracellular expression of CXCR4 and CCR5 in purified primary trophoblast cells from first trimester and term human placentae. Am J Reprod Immunol. 50(4):291-301.

[3] Wiemers, D. O., Ain, R., Ohboshi, S., Soares, M. J. (2003) Migratory trophoblast cells express a newly identified member of the prolactin gene family. J Endocrinol. 179(3):335-46.

[4] Sato, Y., Higuchi, T., Yoshioka, S., Tatsumi, K., Fujiwara, H., Fujii, S. (2003) Trophoblasts acquire a chemokine receptor, CCR1, as they differentiate towards invasive phenotype. Development. 130(22):5519-32.