产品名称：人脊椎间充质干细胞

收到人脊椎间充质干细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人脊椎间充质干细胞Description

Mesenchymal stem cells (MSC) are well-characterized population of ***** stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle [1, 2]. These properties in combination with their developmental plasticity have generated tremendous interest in the potential use of mesenchymal stem cells to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factor will differentiate into chondrocytes, whereas MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC has the capability for renewal and differentiation into various lineages of mesenchymal tissues. In essence MSC could be cultured to expand their numbers then transplanted to the injured site or after seeding in/on shaped biomimetic scaffold to generate appropriate tissue constructs.

HVMSC from ScienCell Research Laboratories are isolated from human vertebral body. HVMSC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HVMSC are characterized by immunofluorescent method with antibodies to CD73, CD90, CD105 and lipid staining after differentiation. HVMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HVMSC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of HVMSC in vitro.

Product Use

HVMSC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

1. Kassem, M. Mesenchymal stem cells: biological characteristics and potential clinical applications. 2004. Cloning Stem Cells. 6(4):369-74.

2. Barry, F. P., and J. M. Murphy. Mesenchymal stem cells: clinical applications and biological characterization. 2004. Int J Biochem Cell Biol. 36(4):568-84.

人脊椎间充质干细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人脊椎间充质干细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人脊椎间充质干细胞其它细胞系展示表：yb-1329 TT(人甲状腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1330 PLC/PRF/5(肝癌亚力山大细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1331 SW579(人甲状腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1332 RKO(结肠腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1333 FaDu(人咽鳞癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1334 NCI-N87(人胃癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1335 DT40(鸡**瘤细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb1000 人脑微血管内皮细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1001 人脑血管平滑肌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1002 人脑血管周细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1003 人脉络丛内皮细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1004 人脉络丛上皮细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1005 人脉络丛成纤维细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1006 人脑膜细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1007 人神经细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1008 人小脑颗粒细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1009 人少突胶质前体细胞- 品牌ATCC 规格25毫升/株. 货期1-2周
贴壁生长 品牌ATCC 规格25毫升/株. 货期1-2周
yb1011 人少突胶质前体细胞- 品牌ATCC 规格25毫升/株. 货期1-2周
悬浮生长 品牌ATCC 规格25毫升/株. 货期1-2周
yb1013 人星形胶质细胞 品牌ATCC 规格25毫升/株. 货期1-2周
yb1014 人小脑星形胶质细胞 品牌ATCC 规格25毫升/株. 货期1-2周