产品名称：小鼠心脏成纤维细胞

收到小鼠心脏成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠心脏成纤维细胞Description

The cardiac fibroblasts (CF) are the principal cell type of the heart. CF provide structural support for cardiac myocytes, are a source of paracrine growth factors, and are an important cellular component of myocardial responses to injury. CF are responsible for extracellular matrix synthesis in the heart during development and pathological conditions. Their proliferation and matrix synthesis is essential for scar formation at sites of myocardial infarction [1]. In addition, cardiac fibroblast proliferation can lead to cardiac fibrosis and contribute to cardiac hypertrophy [2]. CF also respond to changes in microenvironments in pathological conditions by modulating integrin expression [3]. CF culture has been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.

MCF from ScienCell Research Laboratories are isolated from postnatal day 2 mouse heart. MCF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MCF are characterized by immunofluorescence with antibody specific to fibronectin. MCF are negative for mycoplasma, bacteria, yeast, and fungi. MCF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. No. 2331) for the culturing of MCF in vitro.

Product Use

MCF is for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9.

[2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54.

[3] Burgess, M. L., Terracio, L, Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87

小鼠心脏成纤维细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠心脏成纤维细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠心脏成纤维细胞其它细胞系展示表：MPC-83 胰腺腺泡癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C3 白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
RIN-m5F β胰岛素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
L1210 **白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MA891 乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
TA2 自发高乳腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SP2/0 骨髓瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
U14 宫颈癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
YAC-1 **瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
G422 神经胶质瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
TC-1 HPV16，E6,E7和ras基因共转化的C57BL/C(H-2b)小鼠肺上皮细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SRA01/04 人晶体上皮细胞系 品牌ATCC 规格25毫升/株. 货期1-2周
MCF-7 乳腺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CNE-1 高分化鼻咽癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MEF-7/Adr 乳腺腺癌阿霉素耐药株 品牌ATCC 规格25毫升/株. 货期1-2周
KB 口腔表皮样癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SK-BR-3 乳腺腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Hep-2 喉癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A2780 卵巢癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Ec109 食管癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Coc2 卵巢癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MGC-803 胃腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
OVCAR-3 卵巢癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SGC7901 胃低分代腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SK-OV-3 卵巢癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HuTu-80 十二指肠腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SKOV-3/DDP 卵巢癌顺铂耐药株 品牌ATCC 规格25毫升/株. 货期1-2周
Caco-2 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HeLa 宫颈癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CoLo205 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HelaS3 宫颈癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周