产品名称：小鼠脊髓神经元

收到小鼠脊髓神经元时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠脊髓神经元Description

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain is known to contain about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons [2].

RN-sc from ScienCell Research Laboratories are isolated from the E14 rat spinal cord. RN-sc were cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RN-sc are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. RN-sc are negative for mycoplasma, bacteria, yeast and fungi. RN-sc are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use neuronal medium (NM, Cat. No. 1521) for the culturing of RN-dsc in vitro.

Product Use

RN-sc are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.

[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.

小鼠脊髓神经元传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠脊髓神经元倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠脊髓神经元其它细胞系展示表：yb-hxbz-166 耐DDP肺癌细胞 A549/DDP 规格25毫升/株.
yb-hxbz-167 人肺扁平上皮癌细胞 QG-56 规格25毫升/株.
yb-hxbz-168 人高转移肺癌细胞 95-D 规格25毫升/株.
yb-hxbz-169 人巨细胞性肺癌细胞 801-D 规格25毫升/株.
yb-hxbz-170 人肺腺癌细胞 SPC-A1 规格25毫升/株.
yb-hxbz-171 人肺腺癌细胞 LTPEP-a-2 规格25毫升/株.
yb-hxbz-172 人肺腺癌细胞 LTEP-a-2 规格25毫升/株.
yb-hxbz-173 人肺腺癌细胞 H1299 规格25毫升/株.
yb-hxbz-174 人肺鳞癌细胞 SK-MES-1 规格25毫升/株.
yb-hxbz-175 人肺腺癌细胞（胸膜渗出液） Calu-3 规格25毫升/株.
yb-hxbz-176 人肺癌细胞系（未分化） Calu-6 规格25毫升/株.
yb-hxbz-177 人胚肺二倍体细胞 HLF-02 规格25毫升/株.
yb-hxbz-178 人肺癌细胞系 Tul-1 规格25毫升/株.
yb-hxbz-179 人胚肺成纤维细胞 HFL-I 规格25毫升/株.
yb-hxbz-180 人胚肺细胞 MRC-5 规格25毫升/株.
yb-hxbz-181 人胚肺细胞 WI-38 规格25毫升/株.
yb-hxbz-182 人SV－40转化肺成纤维细胞 WI－38AV13 规格25毫升/株.
yb-hxbz-183 人肺癌亚历山大细胞系 PLC/PRF/5 规格25毫升/株.
yb-hxbz-184 人肺巨细胞癌(PG)的低转移亚系 PG-LH7 规格25毫升/株.
yb-hxbz-185 人肺巨细胞癌(PG）的高转移亚系 PG-BE1 规格25毫升/株.
yb-hxbz-186 人肺腺癌细胞系 PG49 规格25毫升/株.
yb-hxbz-187 人肺腺癌细胞 GLC-82 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-hxbz-188 人肺巨细胞癌细胞 HLAmP 规格25毫升/株.
yb-hxbz-189 人肺腺癌细胞（转染TK基因的GLC-82细胞） GLC-82-TK 规格25毫升/株.
yb-hxbz-190 人胚肺成纤维细胞突变癌细胞 Z-HL16C 规格25毫升/株.
yb-hxbz-191 人肺动脉内皮细胞 HPAEC 规格25毫升/株.
yb-hxbz-192 人肺泡上皮细胞 HPAEpiC 规格25毫升/株.
yb-hxbz-193 人胚肺成纤维细胞 CCC-HPF-1 规格25毫升/株.
yb-hxbz-194 人低分化肺腺癌细胞 SK-LU-1 规格25毫升/株.