产品名称：大鼠腹脊髓神经元

收到大鼠腹脊髓神经元时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

大鼠腹脊髓神经元Description

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system. The human brain is known to contain about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons [2].

RN-vsc from ScienCell Research Laboratories are isolated from the E14 rat ventral spinal cord. RN-vsc were cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RN-vsc are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. RNvsc are negative for mycoplasma, bacteria, yeast and fungi. RN-vsc are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use neuronal medium (NM, Cat. No. 1521) for the culturing of RN-vsc in vitro.

Product Use

RN-vsc are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.

[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.

大鼠腹脊髓神经元传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，大鼠腹脊髓神经元倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
大鼠腹脊髓神经元其它细胞系展示表：yb1816 hTGFβ1-ELISA 人TGF-β1Elisa试剂盒 Human TGF-β1 ELISA Kit 96 tests
yb1817 hTNF-ELISA 人TNFElisa试剂盒 Human TNF ELISA Kit 96 tests
yb1818 hTNFR1-ELISA 人TNFsRIElisa试剂盒 Human TNFsRI ELISA Kit 96 tests
yb1819 hTNFsRII-ELISA 人可溶性肿瘤坏死因子受体IIElisa试剂盒 Human Soluble TNFRII ELISA Kit 96 tests
yb1820 hTRAIL-ELISA 人TRAILElisa试剂盒 Human TRAIL ELISA Kit 96 tests
yb1821 huPA-ELISA 人uPA/PLAUElisa试剂盒 Human uPA/PLAU ELISA Kit 96 tests
yb1822 huPAR-ELISA 人uPARElisa试剂盒 Human uPAR ELISA Kit 96 tests
yb1823 hVCAM-ELISA 人VCAM-1Elisa试剂盒 Human VCAM-1 ELISA Kit 96 tests
yb1824 hVEGF-ELISA 人血管内皮生长因子ELISA试剂盒 Human VEGF ELISA Kit 96 tests
yb1825 hVEGFR-ELISA 人VEGFR2/KDRELISA试剂盒 Human VEGFR2/KDR ELISA Kit 96 tests
yb1826 hECad-ELISA 人钙粘附蛋白ELISA试剂盒 Human E-Cadherin ELISA Kit 96 tests
yb1827 hCT1-ELISA 人心营养素-1ELISA试剂盒 Human Cardiotrophin-1 ELISA Kit 96 tests
yb1828 hCD40L-ELISA 人可溶性CD40LELISA试剂盒 Human soluble CD40L ELISA Kit 96 tests
yb1829 hEGVEGF-ELISA 人***腺来源的血管内皮生长因子ELISA试剂盒 Human EG-VEGF ELISA Kit 96 tests
yb1830 hRes-ELISA 人抵抗素ELISAELISA试剂盒 Human Resistin ELISA Kit 96 tests
yb1831 hTNF 人TNFβELISA试剂盒 Human TNFβ ELISA Kit 96 tests
yb1832 hVEGFC-ELISA 人VEGF-CELISA试剂盒 Human VEGF-C ELISA Kit 96 tests
yb1833 hSurv-ELISA 人生存素ELISA试剂盒 Human Survivin ELISA Kit 96 tests
yb1834 hTSP2-ELISA 人TSP2ELISA试剂盒 Human TSP2 ELISA Kit 96 tests
yb1835 hCD105-ELISA 人CD105ELISA试剂盒 Human CD105 ELISA Kit 96 tests
yb1836 hAPP-ELISA 人APP ELISA试剂盒 Human APP ELISA Kit 96 tests
yb1837 hBAFF-ELISA 人 BAFF ELISA试剂盒 Human BAFF ELISA Kit 96 tests
yb1838 hPCad-ELISA 人P-CadherinELISA试剂盒 Human P-Cadherin ELISA Kit 96 tests
yb1839 hCD40-ELISA 人CD40ELISA试剂盒 Human CD40 ELISA Kit 96 tests
yb1840 hNCAM-ELISA 人CD56/NCAM-1ELISA试剂盒 Human CD56/NCAM-1 ELISA Kit 96 tests
yb1841 hcMet-ELISA 人c-Met/HGFRELISA试剂盒 Human c-Met/HGFR ELISA Kit 96 tests
yb1842 hErbB2-ELISA 人ErbB-2/Neu2ELISA试剂盒 Human ErbB-2/Neu2 ELISA Kit 96 tests