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脂蛋白a(Lpa)重组蛋白
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 片段与标签Asp4227~Arg4546 (Accession # P08519) with N-terminal His Tag宿主E.coli
- 内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量39.1kDa
- 实际分子量-(差异分析请参阅说明书)
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 95%
- 等电点-
-
应用SDS-PAGE; WB; ELISA; I
脂蛋白a(Lpa)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
脂蛋白a(Lpa)重组蛋白
Recombinant Lipoprotein, a (Lpa)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
脂蛋白a(Lpa)重组蛋白[ PROPERTIES ]
Residues: Asp4227~Arg4546
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P08519
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.4
Predicted Molecular Mass: 39.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
脂蛋白a(Lpa)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
DCMF GNGKGYRGKK ATTVTGTPCQ EWAAQEPHRH STFIPGTNKW AGLEKNYCRN
PDGDINGPWC YTMNPRKLFD YCDIPLCASS SFDCGKPQVE PKKCPGSIVG GCVAHPHSWP
WQVSLRTRFG KHFCGGTLIS PEWVLTAAHC LKKSSRPSSY KVILGAHQEV NLESHVQEIE
VSRLFLEPTQ ADIALLKLSR PAVITDKVMP ACLPSPDYMV TARTECYITG WGETQGTFGT
GLLKEAQLLV IENEVCNHYK YICAEHLARG TDSCQGDSGG PLVCFEKDKY ILQGVTSWGL
GCARPNKPGV YARVSRFVTW IEGMMR
[ REFERENCES ]
1. McLean J.W., et al. (1987) Nature 330:132-137. 2. Salonen E.-M., et al. (1989) EMBO J. 8:4035-4040. 3. Garner B., et al. (2001) J. Biol. Chem. 276:22200-22208. 4. Mikol V., et al. (1996) J. Mol. Biol. 256:751-761.
