产品名称：肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白

肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白

• 物种Canis familiaris; Canine (Dog，犬)相同的名称，不同的物种。
• 片段与标签Thr36~Gly281 with N-terminal His Tag宿主E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量32.6kDa
• 实际分子量-(差异分析请参阅说明书)
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白

  
  

  Recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

  Organism Species: Canis familiaris; Canine (Dog)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  10th Edition (Revised in Jan, 2014)

  肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白[ PROPERTIES ]

  Residues: Thr36~Gly281

  Tags: Two N-terminal Tags, His-tag and T7-tag

  Accession: F1PWB1

  Host: E. coli

  Purity: >95%

  Endotoxin Level: <1.0EU per 1μg

  (determined by the LAL method).

  Formulation: Supplied as lyophilized form in PBS,

  pH7.4, containing 5% trehalose, 0.01% sarcosyl.

  Predicted isoelectric point: 8.6

  Predicted Molecular Mass: 32.6kDa

  Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  [ USAGE ]

  Reconstitute in sterile PBS, pH7.2-pH7.4.

  [ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles.

  Store at 2-8oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Test: The thermal stability is described by the loss rate of the target

  protein. The loss rate was determined by accelerated thermal degradation test,

  that is, incubate the protein at 37oC for 48h, and no obvious degradation and

  precipitation were observed. (Referring from China Biological Products Standard,

  which was calculated by the Arrhenius equation.) The loss of this protein is less

  than 5% within the expiration date under appropriate storage condition.

  肿瘤坏死因子相关凋亡诱导配体(TRAIL)重组蛋白[ SEQUENCES ]

  The sequence of the target protein is listed below.

  TRELK QMQDKYSQSG IACFLKEDDI PWDPSDEESM NNPCWQVKWQ LRQFVRKMIL

  KTYEETIPTA PEKQLNIPYV VSDRGSQRVA AHITGTSRRS MFPIPSSKND KALGHKINSW

  DSTRKGHSFL NNLHLRNGEL VIHQRGFYYI YSQTYFRFQE PEEIPTGQNR KRNKQMVQYI

  YKHTSYPDPI LLMKSARNSC WSKDSEYGLY SIYQGGIFEL KENDRIFVSV SNEQLIDMDQ

  EASFFGAFLI G