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Human CMA1 (Chymase 1, Mast Cell) Sandwich ELISA (STJE0019811)

Human CMA1 (Chymase 1, Mast Cell) Sandwich ELISA (STJE0019811)
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  • 产品名称:Human CMA1 (Chymase 1, Mast Cell) Sandwich ELISA (STJE0019811)
  • 产品型号:STJE0019811
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human CMA1 (Chymase 1, Mast Cell) Sandwich ELISA (STJE0019811)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This CMA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CMA1
Gene ID: 1215
Uniprot ID: CMA1_HUMAN
Specificity: This kit recognizes Human CMA1 in samples. No significant crossreactivity or interference between Human CMA1 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
Function Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Protein Name Chymase
Alpha-Chymase
Mast Cell Protease I
Database Links Reactome: R-HSA-1433557
Reactome: R-HSA-1592389
Reactome: R-HSA-2022377
Cellular Localisation Secreted
Cytoplasmic Granule
Mast Cell Granules
Alternative ELISA Names Chymase ELISA kit
Alpha-Chymase ELISA kit
Mast Cell Protease I ELISA kit
CMA1 ELISA kit
CYH ELISA kit
CYM ELISA kit
Specificity This kit recognizes Human CMA1 in samples. No significant cross-reactivity or interference between Human CMA1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 98
EDTA plasma (n=8) 87-101 92
Cell culture media (n=8) 93-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 276.09 648.72 2043.14 290.74 605.11 2165.00
Standard deviation 17.45 36.98 68.45 15.67 28.32 105.65
CV (%) 6.32 5.70 3.35 5.39 4.68 4.88
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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