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Human COL10A1 (Collagen alpha-1 (X) chain) CLIA Kit (STJC0000409)
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Human COL10A1 (Collagen alpha-1 (X) chain) CLIA Kit (STJC0000409)
产品资料
Human COL10A1 (Collagen alpha-1 (X) chain) CLIA Kit (STJC0000409)
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产品名称:
Human COL10A1 (Collagen alpha-1 (X) chain) CLIA Kit (STJC0000409)
产品型号:
STJC0000409
产品展商:
ST john's laboratory
产品文档:
无相关文档
简单介绍
Human COL10A1 (Collagen alpha-1 (X) chain) CLIA Kit (STJC0000409)
产品描述
Applications:
CLIA
Reactivity:
Human
Note:
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:
7.5pg/mL
Detection Limit:
12.5~800pg/mL
Short Description:
This human COL10A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction:
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:
3.5h
Detection:
Chemiluminescence
Gene Symbol:
COL10A1
Gene ID:
1300
Uniprot ID:
COAA1_HUMAN
Specificity:
This kit recognizes Human COL10 in samples. No significant cross-reactivity or interference between Human COL10 and analogues was observed.
Sample Type:
Serum, plasma and other biological fluids
Post Translational Modifications
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Protein Name
Collagen Alpha-1(X Chain
Database Links
Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000171
Reactome: R-HSA-8948216
Cellular Localisation
Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names
Collagen Alpha-1(X Chain CLIA kit
COL10A1 CLIA kit
Specificity
This kit recognizes Human COL10 in samples. No significant cross-reactivity or interference between Human COL10 and analogues was observed.
Reproducibility
Both intra-CV and inter-CV are
Information sourced from Uniprot.org
Item Specifications Storage
Micro CLIA Plate (Dismountable)
96T: 8 wells ×12 strips strips
-20℃, 6 months
Reference Standard
96T: 2 vials 48T: 1 vial
-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)
96T: 1 vial, 120 μL 60 μL
-20℃, 6 months
Concentrated HRP Conjugate (100×)
96T: 1 vial, 120 μL 60 μL
-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
2-8°C, 6 months
HRP Conjugate Diluent
1 vial, 14 mL
2-8°C, 6 months
Concentrated Wash Buffer (25×)
1 vial, 30 mL
2-8°C, 6 months
Substrate Reagent A
1 vial, 5 mL
2-8℃ (Protect from light)
Substrate Reagent B
1 vial, 5 mL
2-8℃ (Protect from light)
Plate Sealer
5 pieces
Manual
1 copy
Certificate of Analysis
1 copy
Sample Type Range (%) Average Recovery (%)
Serum(n=8)
88-102
93
EDTA plasma(n=8)
95-112
102
Cell culture media(n=8)
89-101
95
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample
1.00
2.00
3.00
1.00
2.00
3.00
n
20.00
20.00
20.00
20.00
20.00
20.00
Mean (pg/mL)
43.82
76.72
344.80
47.71
77.17
374.85
Standard deviation
4.99
7.84
28.31
5.46
8.52
23.65
CV (%)
11.39
10.22
8.21
11.44
11.04
6.31
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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