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Human COL14A1 (Collagen alpha-1 (XIV) chain) CLIA Kit (STJC0000438)

Human COL14A1 (Collagen alpha-1 (XIV) chain) CLIA Kit (STJC0000438)
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  • 产品名称:Human COL14A1 (Collagen alpha-1 (XIV) chain) CLIA Kit (STJC0000438)
  • 产品型号:STJC0000438
  • 产品展商:ST john's laboratory
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简单介绍
Human COL14A1 (Collagen alpha-1 (XIV) chain) CLIA Kit (STJC0000438)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human COL14A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: COL14A1
Gene ID: 7373
Uniprot ID: COEA1_HUMAN
Specificity: This kit recognizes Human COL14 in samples. No significant cross-reactivity or interference between Human COL14 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. May contain numerous cysteine residues involved in inter- and intramolecular disulfide bonding.
Function Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors.
Protein Name Collagen Alpha-1(Xiv Chain
Undulin
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Collagen Alpha-1(Xiv Chain CLIA kit
Undulin CLIA kit
COL14A1 CLIA kit
UND CLIA kit
Specificity This kit recognizes Human COL14 in samples. No significant cross-reactivity or interference between Human COL14 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 96
EDTA plasma(n=8) 95-106 100
Cell culture media(n=8) 87-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 99.16 197.00 896.62 105.11 203.89 841.72
Standard deviation 11.62 14.74 98.90 11.85 24.14 72.14
CV (%) 11.72 7.48 11.03 11.27 11.84 8.57
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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